ArticleMicrobial cell factories2016
Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Advances in erythritol production through synthetic biology and systems metabolic engineering.Archives of microbiology · 2026Review
- Isolation ofACS synthetic biology · 2025Article
- Strain-Specific Features of Primary Metabolome Characteristic for Extremotolerant/Extremophilic Cyanobacteria Under Long-Term Storage.International journal of molecular sciences · 2025Article
- Protein rational design and modification of erythrose reductase for the improvement of erythritol production in Yarrowia lipolytica.Bioprocess and biosystems engineering · 2024Article
- Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies.World journal of microbiology & biotechnology · 2024Review
- Unlocking the potentials of cyanobacterial photosynthesis for directly converting carbon dioxide into glucose.Nature communications · 2023Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such asACS synthetic biology · 2020Article
- Using osmotic stress to stabilize mannitol production inBiotechnology for biofuels · 2020Article
- Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering.Microbial cell factories · 2018Article
- Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil byEngineering in life sciences · 2018Article
- Deletion ofApplied and environmental microbiology · 2018Article
- Erythritol as sweetener-wherefrom and whereto?Applied microbiology and biotechnology · 2018Review
- Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.Microbial cell factories · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundErythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol is currently based on conversion of glucose by selected fungi. In this study, we describe a biotechnological process to produce erythritol from light and CO2, using engineered Synechocystis sp. PCC6803.
methodsBy functionally expressing codon-optimized genes encoding the erythrose-4-phosphate phosphatase TM1254 and the erythrose reductase Gcy1p, or GLD1, this cyanobacterium can directly convert the Calvin cycle intermediate erythrose-4-phosphate into erythritol via a two-step process and release the polyol sugar in the extracellular medium. Further modifications targeted enzyme expression and pathway intermediates.
conclusionsAfter several optimization steps, the best strain, SEP024, produced up to 2.1 mM (256 mg/l) erythritol, excreted in the medium.
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