Evidence map›Paper›PMID 27059485›Full record

ArticleChinese journal of integrative medicine2016

Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells.

Yu-yan Zhang, Hui-fen Zhou, Jie-hong Yang, Yu He, Xiao-qiang Chen, Katsuyoshi Nishinari, Hao-fang Wan, Hai-tong Wan

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Article in Chinese journal of integrative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yu-yan ZhangResearch Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China.
Hui-fen ZhouResearch Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China.
Jie-hong YangResearch Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China.
Yu HePharmaceutical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China.
Xiao-qiang ChenSchool of Food and Pharmaceutical Engineering, Hubei University of Technology, Wuhan, 430068, China.
Katsuyoshi NishinariSchool of Food and Pharmaceutical Engineering, Hubei University of Technology, Wuhan, 430068, China.
Hao-fang WanPharmaceutical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China.
Hai-tong WanResearch Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China. whtong@126.com.
Hubei University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo observe the effects of Danhong Injection (丹红注射液) and its main components, including daiclzein and hydroxysafflor yellow A (HSYA), on the anticoagulation, fibrinolysis, anti-apoptosis in hypoxia model of vein endothelial cells (VECs).

methodsVECs were prepared and were put in a hypoxia environment, which consisted of mixed gas of 95% N and 5% CO mixed gas, when reached confluent culture. Five groups used different treatments, including normal control group, hypoxia group, daiclzein group, HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection, daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry.

resultsCompared with the normal control group, the floating cells number, PAI activity, ET and MDA contents, and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased, whereas the 6-keto-PGF1α and NO contents, and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group, Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity, regulated PAI and t-PA activities, ET and NO contents, and decreased MDA content and cells apoptosis rate (P<0.05 or P<0.01).

conclusionsDanhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.

Indexed as

6-Ketoprostaglandin F1 alphaAnimalsApoptosisBlood CoagulationCell CountCells, CulturedDrugs, Chinese HerbalEndothelial CellsEndothelinsFactor VIIIFibrinolysisFluorescent Antibody TechniqueHumansInfant, NewbornInjectionsMalondialdehyde6-Ketoprostaglandin F1 alphadanhongDrugs, Chinese HerbalEndothelinsFactor VIIIMalondialdehydeNitric OxidePlasminogen InactivatorsSuperoxide DismutaseTissue Plasminogen ActivatoranticoagulationChinese medicinedaiclzeinDanhong Injectionfibrinolysishydroxysafflor yellow Avein endothelial cells

Identifiers

PMID27059485
OpenAlexW2314193395

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.