ArticleAnnals of botany2016
Transformation of plum plants with a cytosolic ascorbate peroxidase transgene leads to enhanced water stress tolerance.
Article in Annals of botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Physiological and Biochemical Responses of Almond (Plants (Basel, Switzerland) · 2025Article
- Influence of different scion-rootstock combinations on sugars, polyamines, antioxidants and malondialdehyde in grafted grapevines under arid conditions.Frontiers in plant science · 2025Article
- CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding.International journal of molecular sciences · 2023Review
- Differential Response of Phenol Metabolism Associated with Antioxidative Network in Elicited Grapevine Suspension Cultured Cells under Saline Conditions.Antioxidants (Basel, Switzerland) · 2022Article
- Wild grapevines as rootstock regulate the oxidative defense system of in vitro grafted scion varieties under drought stress.PloS one · 2022Article
- The Multiple Roles of Ascorbate in the Abiotic Stress Response of Plants: Antioxidant, Cofactor, and Regulator.Frontiers in plant science · 2021Review
- Ascorbic Acid-The Little-Known Antioxidant in Woody Plants.Antioxidants (Basel, Switzerland) · 2019Review
- Antioxidant Metabolism and Chlorophyll Fluorescence during the Acclimatisation toAntioxidants (Basel, Switzerland) · 2019Article
- GroundnutPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization.International journal of molecular sciences · 2018Review
- The Salt-Stress Response of the Transgenic Plum Line J8-1 and Its Interaction with the Salicylic Acid Biosynthetic Pathway from Mandelonitrile.International journal of molecular sciences · 2018Article
- Reactive oxygen species, antioxidant enzyme activity, and gene expression patterns in a pair of nearly isogenic lines of nicosulfuron-exposed waxy maize (Zea mays L.).Environmental science and pollution research international · 2018Article
- Current achievements and future directions in genetic engineering of European plum (Prunus domestica L.).Transgenic research · 2018Review
- Advancements in Water-Saving Strategies and Crop Adaptation to Drought: A Comprehensive Review.Physiologia plantarumReview
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7 authors.
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Abstract
BACKGROUND AND
aimsWater deficit is the most serious environmental factor limiting agricultural production. In this work, the tolerance to water stress (WS) of transgenic plum lines harbouring transgenes encoding cytosolic antioxidant enzymes was studied, with the aim of achieving the durable resistance of commercial plum trees.
methodsThe acclimatization process was successful for two transgenic lines: line C3-1, co-expressing superoxide dismutase (two copies) and ascorbate peroxidase (one copy) transgenes simultaneously; and line J8-1, harbouring four copies of the cytosolic ascorbate peroxidase gene (cytapx). Plant water relations, chlorophyll fluorescence and the levels of antioxidant enzymes were analysed in both lines submitted to moderate (7 d) and severe (15 d) WS conditions. Additionally, in line J8-1, showing the best response in terms of stress tolerance, a proteomic analysis and determination of the relative gene expression of two stress-responsive genes were carried out. KEY
resultsLine J8-1 exhibited an enhanced stress tolerance that correlated with better photosynthetic performance and a tighter control of water-use efficiency. Furthermore, this WS tolerance also correlated with a higher enzymatic antioxidant capacity than wild-type (WT) and line C3-1 plum plants. On the other hand, line C3-1 displayed an intermediate phenotype between WT plants and line J8-1 in terms of WS tolerance. Under severe WS, the tolerance displayed by J8-1 plants could be due to an enhanced capacity to cope with drought-induced oxidative stress. Moreover, proteomic analysis revealed differences between WT and J8-1 plants, mainly in terms of the abundance of proteins related to carbohydrate metabolism, photosynthesis, antioxidant defences and protein fate.
conclusionsThe transformation of plum plants with cytapx has a profound effect at the physiological, biochemical, proteomic and genetic levels, enhancing WS tolerance. Although further experiments under field conditions will be required, it is proposed that J8-1 plants would be an interesting Prunus rootstock for coping with climate change.
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