ArticleInternational journal of molecular sciences2016
UPLC-MS/MS-Based Profiling of Eicosanoids in RAW264.7 Cells Treated with Lipopolysaccharide.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 21 citations in OpenAlex.
- Eicosanoid-immunometabotypes reveal genotypic links to cervicovaginal pH in progestagen synchronised South African Dohne Merino ewes.Frontiers in veterinary science · 2026Article
- Fecal microbiota transplantation for glaucoma; a potential emerging treatment strategy.Current research in microbial sciences · 2024Review
- The Role of Gut Microbiota in Glaucoma Progression and Other Retinal Diseases.The American journal of pathology · 2023Review
- Tailored Polymer-Based Selective Extraction of Lipid Mediators from Biological Samples.Metabolites · 2021Article
- Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis.Cell metabolism · 2021Article
- Comparative Lipidomic Analysis of Extracellular Vesicles Derived fromInternational journal of molecular sciences · 2020Article
- Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures.International journal of molecular sciences · 2020Review
- Mid-gestation serum lipidomic profile associations with spontaneous preterm birth are influenced by body mass index.PloS one · 2020Article
- Simultaneous LC-MS/MS analysis of eicosanoids and related metabolites in human serum, sputum and BALF.Biomedical chromatography : BMC · 2018Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While both the pro- and anti-inflammatory effects of several eicosanoids have been widely studied, the degree of inflammation in cells that results from various eicosanoids has yet to be comprehensively studied. The objective of this study was to assess the effect of lipopolysaccharide (LPS) treatment on eicosanoid content in RAW264.7 cells. An Ultra performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS)-based profiling method was used to analyze the eicosanoid contents of RAW264.7 cells treated with different LPS concentrations. The profiling data were subjected to statistical analyses, such as principal component analysis (PCA) and hierarchical clustering analysis. LPS treatment increased nitric oxide production and secretion of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, in a concentration-dependent manner. In total, 79 eicosanoids were identified in the cells. RAW264.7 cells treated with different LPS concentrations were well differentiated in the PCA score plot. A heatmap was used to identify the eicosanoids that were up- or down-regulated according to the degree of inflammation and LPS concentration. Thirty-nine eicosanoids were upregulated and seven were down-regulated by LPS treatment in a concentration-dependent manner. Our novel UPLC-MS/MS technique can profile eicosanoids, and can evaluate the correlations between inflammation and eicosanoid metabolism.
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