ArticleInternational journal of molecular sciences2016
Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Chromosome-level assembly and analysis of three hydroxy fatty acid-producing Physaria species.Nature communications · 2025Article
- BnLPAT2 gene regulates oil accumulation in Brassica napus by modulating linoleic and linolenic acid levels in seeds.PloS one · 2025Article
- Lysophosphatidic acid acyltransferase 2 and 5 commonly, but differently, promote seed oil accumulation in Brassica napus.Biotechnology for biofuels and bioproducts · 2022Article
- Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in YeastJournal of fungi (Basel, Switzerland) · 2022Review
- Co-expressingMetabolic engineering communications · 2021Article
- Genetic Engineering of Lesquerella with Increased Ricinoleic Acid Content in Seed Oil.Plants (Basel, Switzerland) · 2021Article
- Transcriptome Analysis and Identification of Lipid Genes inInternational journal of molecular sciences · 2021Article
- Article
- Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds.Plant molecular biology · 2019Article
- Article
- Tri-Hydroxy-Triacylglycerol Is Efficiently Produced by Position-Specific Castor Acyltransferases.Plant physiology · 2019Article
- Tissue-specific differences in metabolites and transcripts contribute to the heterogeneity of ricinoleic acid accumulation in Ricinus communis L. (castor) seeds.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Biotechnology for biofuels · 2019Article
- Molecular mechanism of the extended oil accumulation phase contributing to the high seed oil content for the genotype of tung tree (Vernicia fordii).BMC plant biology · 2018Article
- Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil.Plant biotechnology journal · 2018Article
- A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils.Plant biotechnology journal · 2017Article
- Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves.Plant biotechnology journal · 2017Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%-17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%-14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.
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