ArticlePLoS genetics2016
Gifsy-1 Prophage IsrK with Dual Function as Small and Messenger RNA Modulates Vital Bacterial Machineries.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- P22 Small Noncoding RNAs Are Actively Secreted inNon-coding RNA · 2026Article
- Role of Small Non-Coding RNA in Gram-Negative Bacteria: New Insights and Comprehensive Review of Mechanisms, Functions, and Potential Applications.Molecular biotechnology · 2025Review
- The Role of Bacteriophage-Derived Small RNA Molecules in Bacterial and Phage Interactions.Viruses · 2025Review
- Review
- Diverse Prophage Elements ofCells · 2024Article
- Balanced cell division is secured by two different regulatory sites in OxyS RNA.RNA (New York, N.Y.) · 2024Article
- Early antitermination in the atypical coliphage mEp021 mediated by the Gp17 protein.Archives of virology · 2023Article
- Distinct Subcellular Localization of a Type I CRISPR Complex and the Cas3 Nuclease in Bacteria.Journal of bacteriology · 2022Article
- A Peroxide-Responding sRNA Evolved from a Peroxidase mRNA.Molecular biology and evolution · 2022Article
- RNA binding of Hfq monomers promotes RelA-mediated hexamerization in a limiting Hfq environment.Nature communications · 2021Article
- Early termination of the Shiga toxin transcript generates a regulatory small RNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- AmBio · 2019Article
- Prophages in Salmonella enterica: a driving force in reshaping the genome and physiology of their bacterial host?Molecular microbiology · 2019Review
- Dual-Function RNAs.Microbiology spectrum · 2018Review
- Cross-Regulation between Bacteria and Phages at a Posttranscriptional Level.Microbiology spectrum · 2018Review
- Origin, Evolution, and Loss of Bacterial Small RNAs.Microbiology spectrum · 2018Review
- Translational regulation by bacterial small RNAs via an unusual Hfq-dependent mechanism.Nucleic acids research · 2018Article
- OxyS small RNA induces cell cycle arrest to allow DNA damage repair.The EMBO journal · 2018Article
- Article
- A Novel PhoP/PhoQ Regulation Pathway Modulates the Survival of Extraintestinal PathogenicFrontiers in immunology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While an increasing number of conserved small regulatory RNAs (sRNAs) are known to function in general bacterial physiology, the roles and modes of action of sRNAs from horizontally acquired genomic regions remain little understood. The IsrK sRNA of Gifsy-1 prophage of Salmonella belongs to the latter class. This regulatory RNA exists in two isoforms. The first forms, when a portion of transcripts originating from isrK promoter reads-through the IsrK transcription-terminator producing a translationally inactive mRNA target. Acting in trans, the second isoform, short IsrK RNA, binds the inactive transcript rendering it translationally active. By switching on translation of the first isoform, short IsrK indirectly activates the production of AntQ, an antiterminator protein located upstream of isrK. Expression of antQ globally interferes with transcription termination resulting in bacterial growth arrest and ultimately cell death. Escherichia coli and Salmonella cells expressing AntQ display condensed chromatin morphology and localization of UvrD to the nucleoid. The toxic phenotype of AntQ can be rescued by co-expression of the transcription termination factor, Rho, or RNase H, which protects genomic DNA from breaks by resolving R-loops. We propose that AntQ causes conflicts between transcription and replication machineries and thus promotes DNA damage. The isrK locus represents a unique example of an island-encoded sRNA that exerts a highly complex regulatory mechanism to tune the expression of a toxic protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.