ArticleFly2016
A rapid, gentle and scalable method for dissociation and fluorescent sorting of imaginal disc cells for mRNA sequencing.
Article in Fly, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Distinct endothelial gene responses to acute exercise in skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2025Article
- M1BP is an essential transcriptional activator of oxidative metabolism during Drosophila development.Nature communications · 2023Article
- Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material.Frontiers in zoology · 2022Article
- Differential gene expression analysis identified determinants of cell fate plasticity during radiation-induced regeneration in Drosophila.PLoS genetics · 2022Article
- Paths and pathways that generate cell-type heterogeneity and developmental progression in hematopoiesis.eLife · 2021Article
- Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.Frontiers in cell and developmental biology · 2021Article
- Spectrin couples cell shape, cortical tension, and Hippo signaling in retinal epithelial morphogenesis.The Journal of cell biology · 2020Article
- Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET.Nature communications · 2019Article
- Purification of Low-abundant Cells in the Drosophila Visual System.Journal of visualized experiments : JoVE · 2018Article
- Platforms for Single-Cell Collection and Analysis.International journal of molecular sciences · 2018Review
- Article
- The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling.PLoS genetics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Dissociation of imaginal disc cells has been carried out previously to enable flow cytometry and cell sorting to analyze cell cycle progression, cell size, gene expression, and other aspects of imaginal tissues. However, the lengthy dissociation protocols employed may alter gene expression, cell behavior and overall viability. Here we describe a new rapid and gentle method of dissociating the cells of wing imaginal discs that significantly enhances cell viability and reduces the likelihood of gene expression changes. Furthermore, this method is scalable, enabling collection of large amounts of sample for high-throughput experiments without the need for data-distorting amplifications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.