ArticleBioMed research international2016
A CRISPR-Based Toolbox for Studying T Cell Signal Transduction.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- CAR-T cell therapy: a game-changer in cancer treatment and beyond.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2024Review
- B2M is a Biomarker Associated With Immune Infiltration In High Altitude Pulmonary Edema.Combinatorial chemistry & high throughput screening · 2024Article
- TCR ligand potency differentially impacts PD-1 inhibitory effects on diverse signaling pathways.The Journal of experimental medicine · 2023Article
- A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy.Cell metabolism · 2022Article
- Review
- An Engineered T Cell Receptor Variant Realizes the Limits of Functional Binding Modes.Biochemistry · 2020Article
- Review
- Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.BMC genomics · 2019Article
- Efficient CRISPR/Cas9-Mediated Mutagenesis in Primary Murine T Lymphocytes.Current protocols in immunology · 2019Article
- CRISPR Ethics: Moral Considerations for Applications of a Powerful Tool.Journal of molecular biology · 2019Review
- Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Application of Genome Editing Techniques in Immunology.Archivum immunologiae et therapiae experimentalis · 2018Review
- Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.The Journal of experimental medicine · 2018Article
- Simulation of Stimulation: Cytokine Dosage and Cell Cycle Crosstalk Driving Timing-Dependent T Cell Differentiation.Frontiers in physiology · 2018Article
- Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9.Protein & cell · 2017Review
- GapmeR cellular internalization by macropinocytosis induces sequence-specific gene silencing in human primary T-cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
CRISPR/Cas9 system is a powerful technology to perform genome editing in a variety of cell types. To facilitate the application of Cas9 in mapping T cell signaling pathways, we generated a toolbox for large-scale genetic screens in human Jurkat T cells. The toolbox has three different Jurkat cell lines expressing distinct Cas9 variants, including wild-type Cas9, dCas9-KRAB, and sunCas9. We demonstrated that the toolbox allows us to rapidly disrupt endogenous gene expression at the DNA level and to efficiently repress or activate gene expression at the transcriptional level. The toolbox, in combination with multiple currently existing genome-wide sgRNA libraries, will be useful to systematically investigate T cell signal transduction using both loss-of-function and gain-of-function genetic screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.