ArticleJournal of toxicology2016
Estimation of the Mechanism of Adrenal Action of Endocrine-Disrupting Compounds Using a Computational Model of Adrenal Steroidogenesis in NCI-H295R Cells.
Article in Journal of toxicology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Machine Learning and Large Language Models for Modeling Complex Toxicity Pathways and Predicting Steroidogenesis.Environmental science & technology · 2025Article
- Thiourea-functionalized aminoglutethimide derivatives as anti-leishmanial agents.Future medicinal chemistry · 2024Article
- Towards a microfluidic H295R steroidogenesis assay-biocompatibility study and steroid detection on a thiol-ene-based chip.Analytical and bioanalytical chemistry · 2023Article
- Evaluating structure-based activity in a high-throughput assay for steroid biosynthesis.Computational toxicology (Amsterdam, Netherlands) · 2022Article
- The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells.Postepy dermatologii i alergologii · 2021Article
- Development of a prioritization method for chemical-mediated effects on steroidogenesis using an integrated statistical analysis of high-throughput H295R data.Regulatory toxicology and pharmacology : RTP · 2019Article
- High-Throughput H295R Steroidogenesis Assay: Utility as an Alternative and a Statistical Approach to Characterize Effects on Steroidogenesis.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Identification of candidate reference chemicals for in vitro steroidogenesis assays.Toxicology in vitro : an international journal published in association with BIBRA · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adrenal toxicity is one of the major concerns in drug development. To quantitatively understand the effect of endocrine-active compounds on adrenal steroidogenesis and to assess the human adrenal toxicity of novel pharmaceutical drugs, we developed a mathematical model of steroidogenesis in human adrenocortical carcinoma NCI-H295R cells. The model includes cellular proliferation, intracellular cholesterol translocation, diffusional transport of steroids, and metabolic pathways of adrenal steroidogenesis, which serially involve steroidogenic proteins and enzymes such as StAR, CYP11A1, CYP17A1, HSD3B2, CYP21A2, CYP11B1, CYP11B2, HSD17B3, and CYP19A1. It was reconstructed in an experimental dynamics of cholesterol and 14 steroids from an in vitro steroidogenesis assay using NCI-H295R cells. Results of dynamic sensitivity analysis suggested that HSD3B2 plays the most important role in the metabolic balance of adrenal steroidogenesis. Based on differential metabolic profiling of 12 steroid hormones and 11 adrenal toxic compounds, we could estimate which steroidogenic enzymes were affected in this mathematical model. In terms of adrenal steroidogenic inhibitors, the predicted action sites were approximately matched to reported target enzymes. Thus, our computer-aided system based on systems biological approach may be useful to understand the mechanism of action of endocrine-active compounds and to assess the human adrenal toxicity of novel pharmaceutical drugs.
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Registered trials
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