Evidence map›Paper›PMID 27057121›Full record

ArticleVeterinary world2016

Occurrence of pathogenic Vibrio parahaemolyticus in crustacean shellfishes in coastal parts of Eastern India.

S Parthasarathy, Suresh Chandra Das, Ashok Kumar

Abstract read
In one paragraph

Article in Veterinary world, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens.Applied biochemistry and biotechnology · 2022
    Article
  4. Article
  5. Article
  6. PathogenicHeliyon · 2019
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

S ParthasarathyDivision of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
Suresh Chandra DasVeterinary Public Health Laboratory, Indian Veterinary Research Institute, Eastern Regional Station, Kolkata - 700 037, West Bengal, India.
Ashok KumarAssistant Director General (Animal Health), Indian Council of Agricultural Research, Krishi Bhawan, New Delhi, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimThe objective of the study was to isolate and characterize pathogenic Vibrio parahaemolyticus from crustacean shellfishes (crab and shrimp) commonly retailed in coastal parts of eastern India. MATERIALS AND

methodsSamples were processed by bacteriological isolation followed by biochemical characterization in Kaper's medium. Presumptively identified isolates were confirmed by species-specific Vp-toxR polymerase chain reaction (PCR) assay. Virulence and pandemic property of the confirmed V. parahaemolyticus isolates were determined by specific PCR assays.

resultsOn screening of 167 samples comprising crabs (n=82) and shrimps (n=85) by the standard bacteriological cultural method, V. parahaemolyticus was presumptively identified in 86.6% (71/82) and 82.3% (70/85) of respective samples. Of these, 46 (56%) and 66 (77.6%) isolates from crab and shrimp, respectively, were confirmed as V. parahaemolyticus by biochemical characterization (Kaper's reaction) followed by specific Vp-toxR PCR assay. About 10 isolates each from crab and shrimp was found to carry the virulence gene (tdh). It denotes that 12.2% of crab and 11.7% of shrimp in the study area are harboring the pathogenic V. parahaemolyticus. Such tdh (+) isolates (n=20) were subjected for screening of pandemic genotype by pandemic group specific (PGS) - PCR (PGS-PCR) and GS-PCR (toxRS gene) where 11 (6.5%) isolates revealed the pandemic determining amplicon (235 bp) in PGS-PCR and belonged to crab (7.3%) and shrimp (6%) samples; however, 2 (2.4%) isolates were positive in GS-PCR and belonged to crab samples only. These two GS-PCR(+) isolates from crab were also positive in PGS-PCR.

conclusionThe findings of the study conclusively indicated that a considerable percentage of crab and shrimp in these areas were harboring pathogenic and pandemic V. parahaemolyticus posing a public health risk in consumption of improperly processed such shellfishes. Cross contamination of other marine and fresh water market fishes by such shellfishes in these areas may provide scope for spreading this pathogen in community food chain.

Indexed as

crustaceanspandemicpathogenictoxR-geneVibrio parahaemolyticus

Identifiers

PMID27057121
PMCPMC4823298

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.