Evidence map›Paper›PMID 27056913›Full record

ArticleMolecular & cellular proteomics : MCP2016

Proteomic Insight Reveals Elevated Levels of Albumin in Circulating Immune Complexes in Diabetic Plasma.

Shweta Bhat, Mashanipalya G Jagadeeshaprasad, Yugendra R Patil, Mahemud L Shaikh, Bhaskaran S Regin, Viswanathan Mohan, Ashok P Giri, Muthuswamy Balasubramanyam, Ramanamurthy Boppana, Mahesh J Kulkarni

Open access · hybridAbstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Immunoproteomics: The Evolution from Classical Methods to Emerging Techniques.Methods in molecular biology (Clifton, N.J.) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Shweta BhatFrom the ‡Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune-411008, India, and Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;
Mashanipalya G JagadeeshaprasadFrom the ‡Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune-411008, India, and Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;
Yugendra R PatilFrom the ‡Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune-411008, India, and Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;
Mahemud L Shaikh§National Center for Cell Science, Pune-411007, India;
Bhaskaran S Regin¶Madras Diabetes Research Foundation, Chennai-600 086, India.
Viswanathan Mohan¶Madras Diabetes Research Foundation, Chennai-600 086, India.
Ashok P GiriFrom the ‡Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune-411008, India, and Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;
Muthuswamy Balasubramanyam¶Madras Diabetes Research Foundation, Chennai-600 086, India.
Ramanamurthy Boppana§National Center for Cell Science, Pune-411007, India;
Mahesh J KulkarniFrom the ‡Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune-411008, India, and Academy of Scientific and Innovative Research (AcSIR), New Delhi, India; mj.kulkarni@ncl.res.in.
Academy of Scientific and Innovative Research · INMadras Diabetes Research Foundation · INNational Centre for Cell Science · INNational Chemical Laboratory · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A Hyperglycemic condition in diabetes promotes formation of advanced glycation end products, which are known to elicit immune response and form complexes with immunoglobulins called circulating immune complexes. To investigate the involvement of advanced glycation end product (AGE)-modified proteins in the elicitation of an immune response, circulating immune complexes were isolated and proteins associated were identified and characterized. Label-free-based mass spectrometric analysis of circulating immune complexes in clinical plasma of prediabetic, newly diagnosed diabetes, and diabetic microalbuminurea revealed elevated levels of serum albumin in the circulating immune complexes, which were also observed to be AGE modified. Further, to examine the role of glycation, circulating immune complexeswere analyzed in the streptozotocin-induced diabetic mice treated with or without aminoguanidine, a prototype glycation inhibitor. Mass spectrometric analysis of circulating immune complexes showed elevated levels of serum albumin in plasma from diabetic mice over that of control animals. Aminoguanidine-treated diabetic mice displayed decreased AGE modification of plasma albumin, accompanied by a reduced level of albumin in the circulating immune complexes. In addition, elevated levels of proinflammatory cytokines such as IL-1b, IL-2, and TNF-alpha were observed in diabetes, which were reduced with aminoguanidine treatment, suggesting the involvement of glycation in the immune response.

Indexed as

AnimalsBlood ProteinsCytokinesDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2Gene Expression RegulationGlycation End Products, AdvancedGuanidinesHumansMaleMass SpectrometryMiceProteomicsSerum AlbuminStreptozocinBlood ProteinsCytokinesGlycation End Products, AdvancedGuanidinespimagedineSerum AlbuminStreptozocin

Identifiers

PMID27056913
PMCPMC5083096
OpenAlexW2335803026

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.