ArticleCellular and molecular life sciences : CMLS2016
Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state.
Article in Cellular and molecular life sciences : CMLS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.Biophysical journal · 2025Article
- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.bioRxiv : the preprint server for biology · 2024Article
- Review
- Postsynaptic synucleins mediate endocannabinoid signaling.Nature neuroscience · 2023Article
- SNARE Modulators and SNARE Mimetic Peptides.Biomolecules · 2022Review
- Astrocyte arborization enhances CaGlia · 2021Article
- Exocytosis in Astrocytes.Biomolecules · 2021Review
- The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores.eLife · 2021Article
- TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.Cell reports · 2021Article
- Neurotropic Viruses, Astrocytes, and COVID-19.Frontiers in cellular neuroscience · 2021Review
- Astrocytes, Noradrenaline, α1-Adrenoreceptors, and Neuromodulation: Evidence and Unanswered Questions.Frontiers in cellular neuroscience · 2021Review
- Exocytotic fusion pore under stress.Cell stress · 2020Review
- Exocytosis of large-diameter lysosomes mediates interferon γ-induced relocation of MHC class II molecules toward the surface of astrocytes.Cellular and molecular life sciences : CMLS · 2020Article
- Article
- Article
- Astrocytes monitor cerebral perfusion and control systemic circulation to maintain brain blood flow.Nature communications · 2020Article
- Gliocrine System: Astroglia as Secretory Cells of the CNS.Advances in experimental medicine and biology · 2019Review
- Individual synaptic vesicles mediate stimulated exocytosis from cochlear inner hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation of myelin structure and conduction velocity by perinodal astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Presenilin PS1∆E9 disrupts mobility of secretory organelles in rat astrocytes.Acta physiologica (Oxford, England) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Key support for vesicle-based release of gliotransmitters comes from studies of transgenic mice with astrocyte-specific expression of a dominant-negative domain of synaptobrevin 2 protein (dnSNARE). To determine how this peptide affects exocytosis, we used super-resolution stimulated emission depletion microscopy and structured illumination microscopy to study the anatomy of single vesicles in astrocytes. Smaller vesicles contained amino acid and peptidergic transmitters and larger vesicles contained ATP. Discrete increases in membrane capacitance, indicating single-vesicle fusion, revealed that astrocyte stimulation increases the frequency of predominantly transient fusion events in smaller vesicles, whereas larger vesicles transitioned to full fusion. To determine whether this reflects a lower density of SNARE proteins in larger vesicles, we treated astrocytes with botulinum neurotoxins D and E, which reduced exocytotic events of both vesicle types. dnSNARE peptide stabilized the fusion-pore diameter to narrow, release-unproductive diameters in both vesicle types, regardless of vesicle diameter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.