Evidence map›Paper›PMID 27056271›Full record

ArticleThe Journal of allergy and clinical immunology2016

Sphingosine-1-phosphate/sphingosine-1-phosphate receptor 1 signaling is required for migration of naive human T cells from the thymus to the periphery.

Rachel S Resop, Marc Douaisi, Joshua Craft, Loes C M Jachimowski, Bianca Blom, Christel H Uittenbogaart

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of allergy and clinical immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. From thymus to tissues and tumors: A review of T-cell biology.The Journal of allergy and clinical immunology · 2023
    Review
  6. Article
  7. Review
  8. TargetingCancers · 2021
    Article
  9. Thymus and autoimmunity.Seminars in immunopathology · 2021
    Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Rachel S ResopDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, Calif; UCLA AIDS Institute, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Calif.
Marc DouaisiDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, Calif.
Joshua CraftDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, Calif.
Loes C M JachimowskiAcademic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Bianca BlomAcademic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Christel H UittenbogaartDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, Calif; Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands; UCLA AIDS Institute, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Calif; Department of Pediatrics, University of California Los Angeles, Amsterdam, The Netherlands. Electronic address: uittenbo@ucla.edu.
University of California, Los Angeles · USAcademic Medical Center · NLUniversity of Amsterdam · NL

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ANTON, PETER A · 1991 to 2018
$39.9M
Training in Developmental HematologyT32HL086345 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KOHN, DONALD B · 2007 to 2022
$3.8M
IFN-a and pDC in HIV infection: impact on T cell development and reconstitutionR01AI080564 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI UITTENBOGAART, CHRISTEL H. · 2009 to 2013
$2.0M
HIV-induced immune activation impairs immune reconstitution through S1PR21AI102771 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI UITTENBOGAART, CHRISTEL H. · 2012 to 2013
$384k
NCI NIH HHS P30 CA016042NHLBI NIH HHS T32 HL086345NIAID NIH HHS P30 AI028697NIAID NIH HHS R01 AI080564NIAID NIH HHS R21 AI102771
6 · The paper itself

Abstract

backgroundThe mechanisms that govern the egress of mature thymocytes from the human thymus to the periphery remain understudied yet are of utmost importance to the field of basic immunology, as well as T-cell reconstitution in various immunodeficiencies. We examined the expression and function of sphingosine-1-phosphate (S1P) receptors in human thymocyte egress.

objectivesWe aimed to determine whether S1P receptors (S1P-Rs) play a role in mature human thymocyte egress and to identify the thymocyte population or populations that express S1P-Rs and respond to S1P by migrating across a concentration gradient.

methodsHuman thymocytes were exposed to S1P in Transwell plate migration assays coupled to flow cytometry to evaluate the response to S1P of thymocytes at different stages of maturation. Constitutive S1P-R expression was quantified by means of real-time PCR in sorted thymocyte subsets and flow cytometry. S1P-R1 and Kruppel-like factor 2 expression were monitored after S1P exposure by using flow cytometry and quantitative PCR.

resultsS1P-R1 was the prevalent S1P receptor on mature human thymocytes (CD3(hi)CD27(+)CD69(-)), the population that also demonstrated the greatest response to S1P in migration assays. Pretreatment with FTY720, an S1P-R1 nonselective modulator significantly reduced migration and suggested a role for S1P-R2 in retaining thymocytes in the tissue. Lastly, surface S1P-R1 expression, as well S1PR1 and Kruppel-like factor 2 (KLF2) transcripts, were significantly decreased in mature thymocytes on exposure to S1P.

conclusionMature human thymocytes rely on S1P-R1 to migrate toward S1P. Taken in the context of murine work demonstrating that S1P is required for thymocyte egress to the periphery, our data highlight a new key chemokine for human thymocyte egress.

Indexed as

Signal TransductionBiomarkersCell MovementChemotaxisGene ExpressionHumansImmunophenotypingLysophospholipidsPhenotypeReceptors, LysosphingolipidSphingosineThymocytesThymus GlandT-LymphocytesBiomarkersLysophospholipidsReceptors, LysosphingolipidSphingosinesphingosine 1-phosphatehuman immunologymigrationS1P-R1S1P-R2Sphingosine-1-phosphateT-cell reconstitutionthymic egressthymus

Identifiers

PMID27056271
PMCPMC7007110
OpenAlexW2340261662

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.