Trial reportBMC veterinary research2016
An in vitro method to test the safety and efficacy of low-level laser therapy (LLLT) in the healing of a canine skin model.
Trial report in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Effects of Low-Level Laser Therapy on Oral Mucosal Wound Healing and Systemic Oxidative Stress in Diabetic Rats: An In Vivo Experimental Study.Medicina (Kaunas, Lithuania) · 2025Article
- In Vitro Effects of Low-Level Laser Therapy on the Viability and Secretory Activity of Resting Macrophages.Biomedicines · 2025Article
- Photobiomodulation in Orthodontics: Mechanisms and Clinical Efficacy for Faster Tooth Movement.Cureus · 2024Review
- Effect of red light on epidermal proliferation and mitochondrial activity.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2023Article
- Topical Probiotic Formulation Promotes Rapid Healing in Dog Keratinocyte Cells: A Promising Approach for Wound Management.International journal of molecular sciences · 2023Article
- Photobiomodulation by a new optical fiber device: analysis of the in vitro impact on proliferation/migration of keratinocytes and squamous cell carcinomas cells stressed by X-rays.Lasers in medical science · 2021Article
- Mechanisms of PhotoBioModulation (PBM) focused on oral mucositis prevention and treatment: a scoping review.BMC oral health · 2021Article
- Effects of green light photobiomodulation on Dental Pulp Stem Cells: enhanced proliferation and improved wound healing by cytoskeleton reorganization and cell softening.Lasers in medical science · 2021Article
- Effect of Low-Level Laser Therapy on Bacterial Counts of Contaminated Traumatic Wounds in Dogs.Journal of lasers in medical sciences · 2021Article
- Photobiomodulation therapy improves human dental pulp stem cell viability and migration in vitro associated to upregulation of histone acetylation.Lasers in medical science · 2020Article
- Photobiomodulation effects on keratinocytes cultured in vitro: a critical review.Lasers in medical science · 2019Review
- Anti-inflammatory and cell proliferative effect of the 1270 nm laser irradiation on the BALB/c nude mouse model involves activation of the cell antioxidant system.Biomedical optics express · 2019Article
- Could adverse effects and complications of selective laser trabeculoplasty be decreased by low-power laser therapy?International ophthalmology · 2019Review
- Laser Therapy for Incision Healing in 9 Dogs.Frontiers in veterinary science · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLow-level laser therapy (LLLT) has been used clinically as a treatment modality for a variety of medical conditions including wound-healing processes. It is an attractive and emerging method to enhance wound healing and improve clinical outcomes both in human and veterinary medicine. Despite the fact that the use of LLLT continues to gain in popularity, there is no universally accepted theory that defends all its cellular effects and beneficial biological processes in tissue repair. The present study was designed to evaluate the effect of LLLT on cellular migration and proliferation of cultured canine epidermal keratinocytes (CPEK) in an in vitro wound healing model.
resultsKeratinocyte migration and proliferation were assessed using a scratch migration assay and a proliferation assay, respectively. Fifteen independent replicates were performed for each assay. Canine epidermal keratinocyte cells exposed to LLLT with 0.1, 0.2, and 1.2 J/cm(2) migrated significantly more rapidly (p < 0.03) and showed significantly higher rates of proliferation (p < 0.0001) compared to non-irradiated cells cultured in the same medium and cells exposed to the higher energy dose of 10 J/cm(2). Irradiation with 10 J/cm(2) was characterized by decreased cellular migration and proliferation. These results revealed that LLLT has a measurable, dose-dependent effect on two different aspects of keratinocyte biology in vitro.
conclusionIn this in vitro wound-healing model, LLLT increased cellular migration and proliferation at doses of 0.1, 0.2, and 1.2 J/cm(2) while exposure to 10 J/cm(2) decreased cellular migration and proliferation. These data suggest that the beneficial effects of LLLT in vivo may be due, in part, to effects on keratinocyte behavior.
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