Evidence map›Paper›PMID 27055902›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2016

Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis-Brief Report.

Xiaofeng Chen, Deborah A Howatt, Anju Balakrishnan, Jessica J Moorleghen, Congqing Wu, Lisa A Cassis, Alan Daugherty, Hong Lu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

  1. Direct Vascular Effects of Angiotensin II (A Systematic Short Review).International journal of molecular sciences · 2024
    Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Angiotensinogen and Megalin Interactions Contribute to Atherosclerosis-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2019
    Article
  10. Reporting Sex and Sex Differences in Preclinical Studies.Arteriosclerosis, thrombosis, and vascular biology · 2018
    Article
  11. Renin-Angiotensin System and Cardiovascular Functions.Arteriosclerosis, thrombosis, and vascular biology · 2018
    Review
  12. Review
  13. Article
  14. Association BetweenIn vivo (Athens, Greece)
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xiaofeng ChenFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Deborah A HowattFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Anju BalakrishnanFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Jessica J MoorleghenFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Congqing WuFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Lisa A CassisFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Alan DaughertyFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.).
Hong LuFrom the Laboratory of Cardiovascular Disease, Department of Cardiology, Taizhou Hospital, Wenzhou Medical University, Zhejiang, China (X.C.); Saha Cardiovascular Research Center (X.C., D.A.H., A.B., J.J.M., C.W., A.D., H.L.), Department of Pharmacology and Nutritional Sciences (L.A.C., A.D.), and Department of Physiology, University of Kentucky, Lexington (A.D., H.L.). Hong.Lu@uky.edu.
Wenzhou Medical University · CN

Funding

Sources and effects of angiotensin peptides in atherosclerosisR01HL062846 · NHLBI · UNIVERSITY OF KENTUCKY · PI DAUGHERTY, ALAN · 2000 to 2012
$2.8M
NHLBI NIH HHS R01 HL062846
6 · The paper itself

Abstract

objectiveAngiotensin-converting enzyme (ACE) is present in many cell types of atherosclerotic lesions. This study determined whether ACE activity in endothelial and smooth muscle cells (SMCs), 2 major resident cell types of the aorta, contributes to hypercholesterolemia-induced atherosclerosis. APPROACH AND

resultsAll study mice were in low-density lipoprotein receptor(-/-) background. To determine the contribution of ACE on endothelial cells to atherosclerosis, female ACE floxed mice were bred to male Tie2-Cre transgenic mice. Endothelial cell-specific deletion of ACE significantly decreased serum ACE activity, but had no effect on systolic blood pressure and atherosclerosis. Because ACE protein is present on SMCs, the most abundant cell type of the aorta, we then determined whether ACE on SMCs contributes to atherosclerosis. ACE was depleted from SMCs by breeding female ACE floxed mice with male SM22-Cre transgenic mice. SMC-specific deficiency of ACE did not affect ACE activity in serum, but ablated its presence and activity in the aortic media. Although SMC-specific deficiency of ACE had no effect on systolic blood pressure, it significantly attenuated hypercholesterolemia-induced atherosclerosis in both male and female mice.

conclusionsThese studies provide direct evidence that ACE derived from endothelial cells does not play a critical role in atherosclerosis. Rather, SMC-derived ACE contributes to atherosclerosis, independent of circulating ACE activity and blood pressure.

Indexed as

AnimalsAorta, ThoracicAortic DiseasesAtherosclerosisBlood PressureDisease Models, AnimalDisease ProgressionEndothelial CellsFemaleGenetic Predisposition to DiseaseHypercholesterolemiaMaleMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MusclePeptidyl-Dipeptidase APeptidyl-Dipeptidase AReceptors, LDLangiotensin-converting enzymeangiotensinsatherosclerosisendothelial cellsmyocytes, smooth muscle

Identifiers

PMID27055902
PMCPMC4882263
OpenAlexW2406055892

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.