ArticlePloS one2016
Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Growth Plate Injuries: Advances and Future Directions in Regenerative Medicine.Annals of biomedical engineering · 2026Review
- Engineered Nano-Bio Interfaces for Stem Cell Therapy.Precision chemistry · 2023Review
- Encapsulation of Human Umbilical Cord Mesenchymal Stem Cells in LunaGel Photocrosslinkable Extracellular Matrix and Subcutaneous Transplantation in Mice.Biomedicines · 2023Article
- The Application of Biomaterials in Spinal Cord Injury.International journal of molecular sciences · 2023Review
- Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis.Beilstein journal of nanotechnology · 2022Review
- Adipose-Derived Stem Cells Secretome and Its Potential Application in "Stem Cell-Free Therapy".Biomolecules · 2021Review
- Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells.BioMed research international · 2021Article
- Review
- Nanotechnological Strategies for Osteoarthritis Diagnosis, Monitoring, Clinical Management, and Regenerative Medicine: Recent Advances and Future Opportunities.Current rheumatology reports · 2020Review
- Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages.Arthritis research & therapy · 2020Article
- The Role of Chronic Inflammatory Bone and Joint Disorders in the Pathogenesis and Progression of Alzheimer's Disease.Frontiers in aging neuroscience · 2020Review
- In Vitro Release of Bioactive Bone Morphogenetic Proteins (GDF5, BB-1, and BMP-2) from a PLGA Fiber-Reinforced, Brushite-Forming Calcium Phosphate Cement.Pharmaceutics · 2019Article
- Nanofibers as Bioinstructive Scaffolds Capable of Modulating Differentiation through Mechanosensitive Pathways for Regenerative Engineering.Regenerative engineering and translational medicine · 2019Article
- Synthetic scaffolds for musculoskeletal tissue engineering: cellular responses to fiber parameters.NPJ Regenerative medicine · 2019Review
- Repair of Damaged Articular Cartilage: Current Approaches and Future Directions.International journal of molecular sciences · 2018Review
- Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells.Scientific reports · 2018Article
- Evaluation of Peripheral Blood and Cord Blood Platelet Lysates in Isolation and Expansion of Multipotent Mesenchymal Stromal Cells.Bioengineering (Basel, Switzerland) · 2018Article
- Use of mesenchymal stem cells seeded on the scaffold in articular cartilage repair.Inflammation and regeneration · 2018Review
- Possibility of inhibiting arthritis and joint destruction by SSEA-3 positive cells derived from synovial tissue in rheumatoid arthritis.Regenerative therapy · 2017Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMesenchymal stem cells (MSCs) have immunosuppressive activity and can differentiate into bone and cartilage; and thus seem ideal for treatment of rheumatoid arthritis (RA). Here, we investigated the osteogenesis and chondrogenesis potentials of MSCs seeded onto nano-fiber scaffolds (NFs) in vitro and possible use for the repair of RA-affected joints.
methodsMSCs derived from healthy donors and patients with RA or osteoarthritis (OA) were seeded on poly-lactic-glycolic acid (PLGA) electrospun NFs and cultured in vitro.
resultsHealthy donor-derived MSCs seeded onto NFs stained positive with von Kossa at Day 14 post-stimulation for osteoblast differentiation. Similarly, MSCs stained positive with Safranin O at Day 14 post-stimulation for chondrocyte differentiation. Surprisingly, even cultured without any stimulation, MSCs expressed RUNX2 and SOX9 (master regulators of bone and cartilage differentiation) at Day 7. Moreover, MSCs stained positive for osteocalcin, a bone marker, and simultaneously also with Safranin O at Day 14. On Day 28, the cell morphology changed from a spindle-like to an osteocyte-like appearance with processes, along with the expression of dentin matrix protein-1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE), suggesting possible differentiation of MSCs into osteocytes. Calcification was observed on Day 56. Expression of osteoblast and chondrocyte differentiation markers was also noted in MSCs derived from RA or OA patients seeded on NFs. Lactic acid present in NFs potentially induced MSC differentiation into osteoblasts.
conclusionsOur PLGA scaffold NFs induced MSC differentiation into bone and cartilage. NFs induction process resembled the procedure of endochondral ossification. This finding indicates that the combination of MSCs and NFs is a promising therapeutic technique for the repair of RA or OA joints affected by bone and cartilage destruction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.