ArticlePloS one2016
Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
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- The phenomenon of anhydrobiosis-structural and functional changes in yeast cells.Applied microbiology and biotechnology · 2025Review
- Cold stress enhances cryotolerance inCurrent research in microbial sciences · 2025Article
- Barriers to Effective Cryopreservation of Cell Therapies: Challenges and Emerging Solution.Advances in experimental medicine and biology · 2025Review
- Article
- Biostasis: A Roadmap for Research in Preservation and Potential Revival of Humans.Brain sciences · 2024Review
- From freezing to functioning: cellular strategies of cold-adapted bacteria for surviving in extreme environments.Archives of microbiology · 2024Review
- Preservation of Bacillus subtilis' cellular liquid state at deep sub-zero temperatures in perchlorate brines.Communications biology · 2024Article
- The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes.Frontiers in bioengineering and biotechnology · 2024Article
- Helping proteins come in from the cold: 5 burning questions about cold-active enzymes.BBA advances · 2024Review
- Liquid-Liquid Phase Separation and Protective Protein Aggregates in Bacteria.Molecules (Basel, Switzerland) · 2023Review
- Comprehensive insights on environmental adaptation strategies in Antarctic bacteria and biotechnological applications of cold adapted molecules.Frontiers in microbiology · 2023Review
- Mining of key genes for cold adaptation fromFrontiers in microbiology · 2023Article
- Role of Stress in the Origin of Life.Life (Basel, Switzerland) · 2022Article
- Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties.Molecules (Basel, Switzerland) · 2022Article
- Isolation of novel cold-tolerance genes from rhizosphere microorganisms of Antarctic plants by functional metagenomics.Frontiers in microbiology · 2022Article
- Insights into lactic acid bacteria cryoresistance using FTIR microspectroscopy.Analytical and bioanalytical chemistry · 2022Article
- Bacterial Growth in Brines Formed by the Deliquescence of Salts Relevant to Cold Arid Worlds.Astrobiology · 2022Article
- The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells.PloS one · 2021Article
- Novel perspective on a conventional technique: Impact of ultra-low temperature on bacterial viability and protein extraction.PloS one · 2021Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During cryopreservation ice nucleation and crystal growth may occur within cells or the intracellular compartment may vitrify. Whilst previous literature describes intracellular vitrification in a qualitative manner, here we measure the intracellular vitrification temperature of bacteria and yeasts under conditions relevant to cryopreservation, including the addition of high levels of permeating and nonpermeating additives and the application of rapid rates of cooling. The effects of growth conditions that are known to modify cellular freezing resistance on the intracellular vitrification temperature are also examined. For bacteria a plot of the activity on thawing against intracellular glass transition of the maximally freeze-concentrated matrix (Tg') shows that cells with the lowest value of intracellular Tg' survive the freezing process better than cells with a higher intracellular Tg'. This paper demonstrates the role of the physical state of the intracellular environment in determining the response of microbial cells to preservation and could be a powerful tool to be manipulated to allow the optimization of methods for the preservation of microorganisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.