ArticlePloS one2016
Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- An atypical EF-hand domain containing resistance protein SRC4 boosts plant immunity in a calcium-enhanced manner.The Plant journal : for cell and molecular biology · 2026Article
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cold Stress-Induced (Z)-3-Hexenol and Thymol Enhance Cold Tolerance of Tea Plants by Activating CaPlant biotechnology journal · 2025Article
- A simple and versatile plasma membrane staining method for visualizing living cell morphology in reproductive tissues across diverse plant species.Plant methods · 2025Article
- Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit inFrontiers in plant science · 2021Article
- Adapting techniques for calcium imaging in muscles of adult Brugia malayi.Invertebrate neuroscience : IN · 2020Article
- Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells ofHorticulture research · 2020Article
- Calreticulin is required for calcium homeostasis and proper pollen tube tip growth in Petunia.Planta · 2017Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most direct technique for studying calcium, which is an essential element for pollen tube growth, is Ca2+ imaging. Because membranes are relatively impermeable, the loading of fluorescent Ca2+ probes into plant cells is a challenging task. Thus, we have developed a new method of loading fluo-4 acetoxymethyl ester into cells that uses a cell lysis solution to improve the introduction of this fluorescent dye into pollen tubes. Using this method, the loading times were reduced to 15 min. Furthermore, loading did not have to be performed at low (4°C) temperatures and was successful at room temperature, and pluronic F-127 was not required, which would theoretically allow for the loading of an unlimited number of cells. Moreover, the method can also be used to fluorescently stain root hairs.
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Registered trials
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