ArticleNature protocols2016
Explant culture of adult zebrafish hearts for epicardial regeneration studies.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Pooled it
- Ex vivo imaging reveals neutrophil behaviors in the adult zebrafish heart.Journal of cell science · 2026Article
- Ex vivo functional whole organ in biomedical research: a review.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2025Review
- BMP signaling promotes zebrafish heart regeneration via alleviation of replication stress.Nature communications · 2025Article
- Advancing ex vivo functional whole-organ prostate gland model for regeneration and drug screening.Scientific reports · 2025Article
- Macrophages and cardiac lesion in zebrafish: what can single-cell RNA sequencing reveal?Frontiers in cardiovascular medicine · 2025Review
- Cardiomyocyte proliferation and heart regeneration in adult Xenopus tropicalis evidenced by a transgenic reporter line.NPJ Regenerative medicine · 2024Article
- Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells.Science advances · 2024Article
- Sinus venosus adaptation models prolonged cardiovascular disease and reveals insights into evolutionary transitions of the vertebrate heart.Nature communications · 2023Article
- Missense Mutation in Human CHD4 Causes Ventricular Noncompaction by Repressing ADAMTS1.Circulation research · 2023Article
- Hooked on heart regeneration: the zebrafish guide to recovery.Cardiovascular research · 2022Review
- Hypoxia promotes a perinatal-like progenitor state in the adult murine epicardium.Scientific reports · 2022Article
- Activation of Nkx2.5 transcriptional program is required for adult myocardial repair.Nature communications · 2022Article
- Visualization of regenerating and repairing hearts.Clinical science (London, England : 1979) · 2022Article
- Intestine Explants in Organ Culture: A Tool to Broaden the Regenerative Studies in Echinoderms.Journal of marine science and engineering · 2022Article
- Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo.Development (Cambridge, England) · 2021Article
- Fosl1 is vital to heart regeneration upon apex resection in adult Xenopus tropicalis.NPJ Regenerative medicine · 2021Article
- The Last Half Century of Fish Explant and Organ Culture.Zebrafish · 2021Article
- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract.Development (Cambridge, England) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe how to culture adult zebrafish hearts as explants and study the regeneration of epicardial tissue ex vivo, as a means to identify therapeutic targets for heart disease. Uninjured or injured adult hearts are excised, washed and cultured in an incubator with gentle agitation. Heart explants can be prepared within 2 h, and they can be maintained in culture for 30 d or longer. If explants are prepared from appropriate transgenic lines, dynamic behaviors of epicardial cells can be monitored by live imaging using stereofluorescence microscopy. We also describe ex vivo procedures for genetic ablation of the epicardium, cell proliferation assays, tissue grafts and bead grafts. Basic cell culture and surgical skills are required to carry out this protocol. Unlike existing protocols for culturing isolated zebrafish epicardial cells on matrices, procedures described here maintain epicardial cells on an intact cardiac surface, thereby better supporting in vivo cell behaviors. Our protocols complement and extend in vivo studies of heart regeneration.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.