Evidence map›Paper›PMID 27053953›Full record

ArticleJournal of Cancer2016

Neutrophil Granulocytes in Ovarian Cancer - Induction of Epithelial-To-Mesenchymal-Transition and Tumor Cell Migration.

Christine Mayer, Silvia Darb-Esfahani, Anne-Sophie Meyer, Katrin Hübner, Joachim Rom, Christof Sohn, Ioana Braicu, Jalid Sehouli, G Maria Hänsch, Matthias M Gaida

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In one paragraph

Article in Journal of Cancer, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  13. Prognostic impact of tumor-associated neutrophils in breast cancer.International journal of clinical and experimental pathology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Christine Mayer1. Department of Gynecology and Obstetrics, University Hospital Heidelberg, Germany;
Silvia Darb-Esfahani2. Institute of Pathology, Charité, University Hospital Berlin, Germany;; 3. Tumorbank Ovarian Cancer Network (TOC), Berlin, Germany;
Anne-Sophie Meyer4. Institute of Pathology, University Hospital Heidelberg, Germany;
Katrin Hübner5. BioQuant, COS Heidelberg, University Heidelberg, Germany;
Joachim Rom1. Department of Gynecology and Obstetrics, University Hospital Heidelberg, Germany;
Christof Sohn1. Department of Gynecology and Obstetrics, University Hospital Heidelberg, Germany;
Ioana Braicu3. Tumorbank Ovarian Cancer Network (TOC), Berlin, Germany;; 6. Department of Gynecology, Charité University Hospital Berlin, Germany;
Jalid Sehouli3. Tumorbank Ovarian Cancer Network (TOC), Berlin, Germany;; 6. Department of Gynecology, Charité University Hospital Berlin, Germany;
G Maria Hänsch7. Institute of Immunology, University Hospital Heidelberg, Germany.
Matthias M Gaida4. Institute of Pathology, University Hospital Heidelberg, Germany;

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOvarian cancer (OvCa) is a highly aggressive malignoma with a tumor-promoting microenvironment. Infiltration of polymorphonuclear neutrophils (PMN) is frequently seen, raising the question of their impact on tumor development. In that context, effects of PMN on human ovarian cancer cells were assessed.

methodsHuman epithelial ovarian cancer cells were incubated with human PMN, lysate of PMN, or neutrophil elastase. Morphological alterations were observed by time-lapse video-microscopy, and the underlying molecular mechanism was analyzed by flow cytometry and Western blotting. Functional alternations were assessed by an in vitro wound healing assay. In parallel, a large cohort of n=334 primary OvCa tissue samples of various histological subtypes was histologically evaluated.

resultsCo-cultivation of cancer cells with either PMN or PMN lysate causes a change of the polygonal epithelial phenotype of the cells towards a spindle shaped morphology, causing a cribriform cell growth. The PMN-induced alteration could be attributed to elastase, a major protease of PMN. Elastase-induced shape change was most likely due to the degradation of membranous E-cadherin, which results in loss of cell contacts and polarity. Moreover, in response to elastase, epithelial cytokeratins were downmodulated, in parallel with a nuclear translocation of β-catenin. These PMN-elastase induced alterations of cells are compatible with an epithelial-to-mesenchymal transition (EMT) of the cancer cells. Following EMT, the cells displayed a more migratory phenotype. In human biopsies, neutrophil infiltration was seen in 72% of the cases. PMN infiltrates were detected preferentially in areas with low E-cadherin expression.

conclusionPMN in the microenvironment of OvCa can alter tumor cells towards a mesenchymal and migratory phenotype.

Indexed as

epithelial to mesenchymal transitionmigration.neutrophil elastaseneutrophilsOvarian cancertumor microenvironment

Identifiers

PMID27053953
PMCPMC4820731

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.