ArticleThe Plant cell2016
Spontaneous Chloroplast Mutants Mostly Occur by Replication Slippage and Show a Biased Pattern in the Plastome of Oenothera.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
- Chloroplast- and mitochondrion-specific random C-to-T mutagenesis for forward genetics of organelle genomes.The Plant journal : for cell and molecular biology · 2026Article
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- Refined chloroplast annotations, repeat profiles, and phylogenomic evidence reveal maternal lineage shifts and independent evolution in the Triticum-Aegilops complex.BMC plant biology · 2026Article
- Complex plastome structural variation caused by intermolecular and intramolecular recombination accounts for leaf variegation inPlant diversity · 2026Article
- Comparative and phylogenetic analysis of chloroplast genomes in the subtribe Leptoboeinae (Gesneriaceae).Frontiers in plant science · 2026Article
- The evolutionary dynamics of organellar pan-genomes in Arabidopsis thaliana.Genome biology · 2025Article
- Flipping the switch on some of the slowest mutating genomes: Direct measurements of plant mitochondrial and plastid mutation rates in msh1 mutants.PLoS genetics · 2025Article
- Novel perspectives on plastome evolution in Onagraceae.AoB PLANTS · 2025Article
- Comparative plastome analyses and phylogenetic insights of Elatostema.BMC plant biology · 2025Article
- Flipping the switch on some of the slowest mutating genomes: Direct measurements of plant mitochondrial and plastid mutation rates inbioRxiv : the preprint server for biology · 2025Article
- Chloroplast genome characteristic, comparative and phylogenetic analyses in Capsicum (Solanaceae).BMC genomics · 2024Article
- Sequence characteristics, genetic diversity and phylogenetic analysis of the Cucurbita ficifolia (Cucurbitaceae) chloroplasts genome.BMC genomics · 2024Article
- Leaf variegation caused by plastome structural variation: an example fromHorticulture research · 2024Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2024Article
- High-throughput discovery of plastid genes causing albino phenotypes in ornamental chimeric plants.Horticulture research · 2023Article
- Long-read sequencing characterizes mitochondrial and plastid genome variants in Arabidopsis msh1 mutants.The Plant journal : for cell and molecular biology · 2022Article
- Sorting of mitochondrial and plastid heteroplasmy inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Epimutations and mutations, nurturing phenotypic diversity.Genetica · 2022Review
- Graph-based models of theNAR genomics and bioinformatics · 2022Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spontaneous plastome mutants have been used as a research tool since the beginning of genetics. However, technical restrictions have severely limited their contributions to research in physiology and molecular biology. Here, we used full plastome sequencing to systematically characterize a collection of 51 spontaneous chloroplast mutants in Oenothera (evening primrose). Most mutants carry only a single mutation. Unexpectedly, the vast majority of mutations do not represent single nucleotide polymorphisms but are insertions/deletions originating from DNA replication slippage events. Only very few mutations appear to be caused by imprecise double-strand break repair, nucleotide misincorporation during replication, or incorrect nucleotide excision repair following oxidative damage. U-turn inversions were not detected. Replication slippage is induced at repetitive sequences that can be very small and tend to have high A/T content. Interestingly, the mutations are not distributed randomly in the genome. The underrepresentation of mutations caused by faulty double-strand break repair might explain the high structural conservation of seed plant plastomes throughout evolution. In addition to providing a fully characterized mutant collection for future research on plastid genetics, gene expression, and photosynthesis, our work identified the spectrum of spontaneous mutations in plastids and reveals that this spectrum is very different from that in the nucleus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.