ArticleScientific reports2016
A systematic analysis of the role of GGDEF-EAL domain proteins in virulence and motility in Xanthomonas oryzae pv. oryzicola.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- High-quality complete genome sequence ofMicrobiology resource announcements · 2023Article
- Sequence Conservation, Domain Architectures, and Phylogenetic Distribution of the HD-GYP Type c-di-GMP Phosphodiesterases.Journal of bacteriology · 2022Review
- Functional Annotation of Hypothetical Proteins From theBioinformatics and biology insights · 2022Article
- Secrete or perish: The role of secretion systems inComputational and structural biotechnology journal · 2021Review
- Comparative Genomics ofInternational journal of molecular sciences · 2020Article
- Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas.FEMS microbiology reviews · 2020Review
- Zinc binding proteome of a phytopathogenRoyal Society open science · 2019Article
- Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate.Applied and environmental microbiology · 2019Article
- Identification of c-di-GMP Signaling Components inFrontiers in microbiology · 2019Review
- Regulation of Protein Secretion Systems Mediated by Cyclic Diguanylate in Plant-Interacting Bacteria.Frontiers in microbiology · 2019Review
- In silico comparative analysis of GGDEF and EAL domain signaling proteins from the Azospirillum genomes.BMC microbiology · 2018Article
- AmrZ is a major determinant of c-di-GMP levels in Pseudomonas fluorescens F113.Scientific reports · 2018Article
- A c-di-GMP-Modulating Protein Regulates Swimming Motility ofFrontiers in cellular and infection microbiology · 2018Article
- A Symphony of Cyclases: Specificity in Diguanylate Cyclase Signaling.Annual review of microbiology · 2017Review
- Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family.BMC genomics · 2017Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The second messenger c-di-GMP is implicated in regulation of various aspects of the lifestyles and virulence of Gram-negative bacteria. Cyclic di-GMP is formed by diguanylate cyclases with a GGDEF domain and degraded by phosphodiesterases with either an EAL or HD-GYP domain. Proteins with tandem GGDEF-EAL domains occur in many bacteria, where they may be involved in c-di-GMP turnover or act as enzymatically-inactive c-di-GMP effectors. Here, we report a systematic study of the regulatory action of the eleven GGDEF-EAL proteins in Xanthomonas oryzae pv. oryzicola, an important rice pathogen causing bacterial leaf streak. Mutational analysis revealed that XOC_2335 and XOC_2393 positively regulate bacterial swimming motility, while XOC_2102, XOC_2393 and XOC_4190 negatively control sliding motility. The ΔXOC_2335/XOC_2393 mutant that had a higher intracellular c-di-GMP level than the wild type and the ΔXOC_4190 mutant exhibited reduced virulence to rice after pressure inoculation. In vitro purified XOC_4190 and XOC_2102 have little or no diguanylate cyclase or phosphodiesterase activity, which is consistent with unaltered c-di-GMP concentration in ΔXOC_4190. Nevertheless, both proteins can bind to c-di-GMP with high affinity, indicating a potential role as c-di-GMP effectors. Overall our findings advance understanding of c-di-GMP signaling and its links to virulence in an important rice pathogen.
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