ArticleScientific reports2016
Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Towards biotechnological production of bio-based low molecular weight esters: a patent review.RSC advances · 2024Review
- Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel.AMB Express · 2024Article
- Article
- A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli.Biotechnology and bioengineering · 2022Article
- Development of NIR-Based ANN Models forMicromachines · 2022Article
- Carboxyl Methyltransferase Catalysed Formation of Mono- and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis.Angewandte Chemie (International ed. in English) · 2022Article
- Escherichia coli coculture for de novo production of esters derived of methyl-branched alcohols and multi-methyl branched fatty acids.Microbial cell factories · 2022Article
- Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification.Bioresources and bioprocessing · 2021Review
- Isobutanol production freed from biological limits using synthetic biochemistry.Nature communications · 2020Article
- Engineering Yarrowia lipolytica for the production of cyclopropanated fatty acids.Journal of industrial microbiology & biotechnology · 2018Article
- Microbial synthesis of medium-chain chemicals from renewables.Nature biotechnology · 2017Review
- Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing.Journal of industrial microbiology & biotechnology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most biodiesel currently in use consists of fatty acid methyl esters (FAMEs) produced by transesterification of plant oils with methanol. To reduce competition with food supplies, it would be desirable to directly produce biodiesel in microorganisms. To date, the most effective pathway for the production of biodiesel in bacteria yields fatty acid ethyl esters (FAEEs) at up to ~1.5 g/L. A much simpler route to biodiesel produces FAMEs by direct S-adenosyl-L-methionine (SAM) dependent methylation of free fatty acids, but FAME production by this route has been limited to only ~16 mg/L. Here we employ an alternative, broad spectrum methyltransferase, Drosophila melanogaster Juvenile Hormone Acid O-Methyltransferase (DmJHAMT). By introducing DmJHAMT in E. coli engineered to produce medium chain fatty acids and overproduce SAM, we obtain medium chain FAMEs at titers of 0.56 g/L, a 35-fold increase over titers previously achieved. Although considerable improvements will be needed for viable bacterial production of FAMEs and FAEEs for biofuels, it may be easier to optimize and transport the FAME production pathway to other microorganisms because it involves fewer enzymes.
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