ArticleExperimental animals2016
Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice.
Article in Experimental animals, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 26 citations in OpenAlex.
- Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters.Cell reports methods · 2026Article
- Lysophosphatidic acid-mediated NF-κB activation promotes FOXC2 expression essential for lymphatic valve development.The Journal of clinical investigation · 2026Article
- Activity of estrogen receptor β expressing neurons in the medial amygdala regulates preference toward receptive females in male mice.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- EpiCRISPR targeted methylation of Arx gene initiates transient switch of mouse pancreatic alpha to insulin-producing cells.Frontiers in endocrinology · 2023Article
- NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.Scientific reports · 2022Article
- Article
- Adipose-specific C-C motif chemokine ligand (CCL) 19 overexpression drives the mice to both insulin resistance and weight gain.BMJ open diabetes research & care · 2021Article
- Characterization of a bicistronic knock-in reporter mouse model for investigating the role of CABLES2 in vivo.Experimental animals · 2021Article
- Loss of β-cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation.Communications biology · 2020Article
- Ins1-Cre and Ins1-CreER Gene Replacement Alleles Are Susceptible To Silencing By DNA Hypermethylation.Endocrinology · 2020Article
- Uncovering the role of MAFB in glucagon production and secretion in pancreatic α-cells using a new α-cell-specific Mafb conditional knockout mouse model.Experimental animals · 2020Article
- Article
- Generation of bicistronic reporter knockin mice for visualizing germ layers.Experimental animals · 2019Article
- MafB Is Important for Pancreatic β-Cell Maintenance under a MafA-Deficient Condition.Molecular and cellular biology · 2019Article
- Article
- Gene-edited CRISPy Critters for alcohol research.Alcohol (Fayetteville, N.Y.) · 2019Review
- Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.Scientific reports · 2017Article
- Simple generation of hairless mice for in vivo imaging.Experimental animals · 2017Article
- Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we generated novel cre driver mice for gene manipulation in pancreatic β cells. Using the CRISPR/Cas9 system, stop codon sequences of Ins1 were targeted for insertion of cre, including 2A sequences. A founder of C57BL/6J-Ins1(em1 (cre) Utr) strain was produced from an oocyte injected with pX330 containing the sequences encoding gRNA and Cas9 and a DNA donor plasmid carrying 2A-cre. (R26GRR x C57BL/6J-Ins1(em1 (cre) Utr)) F1 mice were histologically characterized for cre-loxP recombination in the embryonic and adult stages; cre-loxP recombination was observed in all pancreatic islets examined in which almost all insulin-positive cells showed tdsRed fluorescence, suggesting β cell-specific recombination. Furthermore, there were no significant differences in results of glucose tolerance test among genotypes (homo/hetero/wild). Taken together, these observations indicated that C57BL/6J-Ins1(em1 (cre) Utr) is useful for studies of glucose metabolism and the strategy of bicistronic cre knock-in using the CRISPR/Cas9 system could be useful for production of cre driver mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.