ArticleScientific reports2016
Enoyl-CoA hydratase mediates polyhydroxyalkanoate mobilization in Haloferax mediterranei.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- Endocrine and enzymatic shifts during insect diapause: a review of regulatory mechanisms.Frontiers in physiology · 2025Review
- Transcriptomic profiling of haloarchaeal denitrification through RNA-Seq analysis.Applied and environmental microbiology · 2024Article
- Bioplastic Production from Agri-Food Waste through the Use ofMicroorganisms · 2024Review
- Overproduction ofJournal of microbiology and biotechnology · 2024Article
- Unveiling the repressive mechanism of a PPS-like regulator (PspR) in polyhydroxyalkanoates biosynthesis network.Applied microbiology and biotechnology · 2024Article
- Response of the Anaerobic Methanotrophic ArchaeonFrontiers in microbiology · 2022Article
- Evaluating haloarchaeal culture media for ultrahigh-molecular-weight polyhydroxyalkanoate biosynthesis by Haloferax mediterranei.Applied microbiology and biotechnology · 2021Article
- Subzero, saline incubations of Colwellia psychrerythraea reveal strategies and biomarkers for sustained life in extreme icy environments.Environmental microbiology · 2021Article
- Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Insights into the metabolism pathway and functional genes of long-chain aliphatic alkane degradation in haloarchaea.Extremophiles : life under extreme conditions · 2020Article
- Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory.Microbial cell factories · 2020Review
- Article
- Unusual Phosphoenolpyruvate (PEP) Synthetase-Like Protein Crucial to Enhancement of Polyhydroxyalkanoate Accumulation in Haloferax mediterranei Revealed by Dissection of PEP-Pyruvate Interconversion Mechanism.Applied and environmental microbiology · 2019Article
- Time-Course Proteomic Analysis ofPolymers · 2019Article
- New Insights into PhaM-PhaC-Mediated Localization of Polyhydroxybutyrate Granules in Ralstonia eutropha H16.Applied and environmental microbiology · 2017Article
- Genome Sequence of the Poly-3-Hydroxybutyrate Producer Clostridium acetireducens DSM 10703.Genome announcements · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although polyhydroxyalkanoate (PHA) accumulation and mobilization are one of the most general mechanisms for haloarchaea to adapt to the hypersaline environments with changeable carbon sources, the PHA mobilization pathways are still not clear for any haloarchaea. In this study, the functions of five putative (R)-specific enoyl-CoA hydratases (R-ECHs) in Haloferax mediterranei, named PhaJ1 to PhaJ5, respectively, were thoroughly investigated. Through gene deletion and complementation, we demonstrated that only certain of these ECHs had a slight contribution to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) biosynthesis. But significantly, PhaJ1, the only R-ECH that is associated with PHA granules, was shown to be involved in PHA mobilization in this haloarchaeon. PhaJ1 catalyzes the dehydration of (R)-3-hydroxyacyl-CoA, the common product of PHA degradation, to enoyl-CoA, the intermediate of the β-oxidation cycle, thus could link PHA mobilization to β-oxidation pathway in H. mediterranei. This linkage was further indicated from the up-regulation of the key genes of β-oxidation under the PHA mobilization condition, as well as the obvious inhibition of PHA degradation upon inhibition of the β-oxidation pathway. Interestingly, 96% of phaJ-containing haloarchaeal species possess both phaC (encoding PHA synthase) and the full set genes of β-oxidation, implying that the mobilization of carbon storage in PHA through the β-oxidation cycle would be general in haloarchaea.
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