ArticleBMC microbiology2016
A method for culturing Gram-negative skin microbiota.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Detecting microbial engraftment after FMT using placebo sequencing and culture enriched metagenomics to sort signals from noise.Nature communications · 2025Trial
- A Randomized, Double-Blind, Placebo-Controlled, Multicenter, 16-Week Trial to Evaluate the Efficacy and Safety of FB-401 in Children, Adolescents, and Adult Subjects (Ages 2 Years and Older) with Mild-to-Moderate Atopic Dermatitis.Dermatology (Basel, Switzerland) · 2024Trial
- Therapeutic responses toScience translational medicine · 2020Trial
- TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome.The Journal of clinical investigation · 2018Trial
- First-in-human topical microbiome transplantation with Roseomonas mucosa for atopic dermatitis.JCI insight · 2018Trial
- Review
- Microecology in vitro model replicates the human skin microbiome interactions.Nature communications · 2025Article
- Environmental and behavioral mitigation strategies for patients with atopic dermatitis.JAAD international · 2024Article
- Valorisation of tomato pomace in anti-pollution and microbiome-balance face cream.Scientific reports · 2024Article
- Contribution of the patient microbiome to surgical site infection and antibiotic prophylaxis failure in spine surgery.Science translational medicine · 2024Article
- Integrative Multiomics Approach to Skin: The Sinergy between Individualised Medicine and Futuristic Precision Skin Care?Metabolites · 2024Review
- Article
- Article
- Living symbiotic bacteria-involved skin dressing to combat indigenous pathogens for microbiome-based biotherapy toward atopic dermatitis.Bioactive materials · 2023Article
- Exposure to isocyanates predicts atopic dermatitis prevalence and disrupts therapeutic pathways in commensal bacteria.Science advances · 2023Article
- Emerging Paradigms in the Prevention of Surgical Site Infection: The Patient Microbiome and Antimicrobial Resistance.Anesthesiology · 2022Article
- Integrating cultivation and metagenomics for a multi-kingdom view of skin microbiome diversity and functions.Nature microbiology · 2022Article
- Article
- Cultivation of common bacterial species and strains from human skin, oral, and gut microbiota.BMC microbiology · 2021Article
- Assessing the effects of common topical exposures on skin bacteria associated with atopic dermatitis.Skin health and disease · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCommensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described.
resultsThrough the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures.
conclusionsOur protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.