ArticleScientific reports2016
Characterization of a temperature-responsive two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- A novel two-component system regulates shikimate pathway for thermal adaptation in Thermus thermophilus.Nucleic acids research · 2026Article
- A Two-Component Regulatory System Mediates Quorum Sensing-Dependent Morphology and Motility Transitions in the ArchaeonbioRxiv : the preprint server for biology · 2025Article
- Archaeal Signalling Networks-New Insights Into the Structure and Function of Histidine Kinases and Response Regulators of the Methanogenic Archaeon Methanosarcina acetivorans.Environmental microbiology · 2025Article
- Advances in Extremophile Research: Biotechnological Applications through Isolation and Identification Techniques.Life (Basel, Switzerland) · 2024Review
- Taxonomic identification and temperature stress tolerance mechanisms of Aequorivita marisscotiae sp. nov.Communications biology · 2023Article
- Transcriptional factor OmpR positively regulates prodigiosin biosynthesis inFrontiers in microbiology · 2022Article
- The Journey from Two-Step to Multi-Step Phosphorelay Signaling Systems.Current genomics · 2021Review
- Identification of Novel Thermosensors in Gram-Positive Pathogens.Frontiers in molecular biosciences · 2020Article
- What do archaeal and eukaryotic histidine kinases sense?F1000Research · 2019Review
- A vision for a 'microbcentric' future.Microbial biotechnology · 2019Article
- Phyletic Distribution and Lineage-Specific Domain Architectures of Archaeal Two-Component Signal Transduction Systems.Journal of bacteriology · 2018Article
- Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR.Scientific reports · 2017Article
- Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: investigating acetamidase gene function.Scientific reports · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cold environments dominate the Earth's biosphere and the resident microorganisms play critical roles in fulfilling global biogeochemical cycles. However, only few studies have examined the molecular basis of thermosensing; an ability that microorganisms must possess in order to respond to environmental temperature and regulate cellular processes. Two component regulatory systems have been inferred to function in thermal regulation of gene expression, but biochemical studies assessing these systems in Bacteria are rare, and none have been performed in Archaea or psychrophiles. Here we examined the LtrK/LtrR two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii, assessing kinase and phosphatase activities of wild-type and mutant proteins. LtrK was thermally unstable and had optimal phosphorylation activity at 10 °C (the lowest optimum activity for any psychrophilic enzyme), high activity at 0 °C and was rapidly thermally inactivated at 30 °C. These biochemical properties match well with normal environmental temperatures of M. burtonii (0-4 °C) and the temperature this psychrophile is capable of growing at in the laboratory (-2 to 28 °C). Our findings are consistent with a role for LtrK in performing phosphotransfer reactions with LtrR that could lead to temperature-dependent gene regulation.
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