ArticleScientific reports2016
Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Enhancement of levopimaradiene production inChinese herbal medicines · 2026Article
- Advances in biomanufacturing and recent biological applications of the fundamental bacterial carotenoids: a comprehensive review.Microbial cell factories · 2025Review
- Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons.Nature communications · 2025Article
- Evolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction.Nature communications · 2024Article
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- Efficient enzymatic synthesis of chiral 2,3-dihydro-1,4-benzodioxane motif using engineeredRSC advances · 2023Article
- EngineeringSynthetic and systems biotechnology · 2022Review
- Functional Characterization and Screening of Promiscuous Kinases and Isopentenyl Phosphate Kinases for the Synthesis of DMAPP via a One-Pot Enzymatic Cascade.International journal of molecular sciences · 2022Article
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- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
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- Review
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- Diversifying Isoprenoid PlatformsFrontiers in microbiology · 2021Review
- Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids.Journal of industrial microbiology & biotechnology · 2020Review
- Probing the Substrate Promiscuity of Isopentenyl Phosphate Kinase as a Platform for Hemiterpene Analogue Production.Chembiochem : a European journal of chemical biology · 2019Article
- The isoprenoid alcohol pathway, a synthetic route for isoprenoid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Exploring natural biodiversity to expand access to microbial terpene synthesis.Microbial cell factories · 2019Article
- Two-step pathway for isoprenoid synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Protein rational design has become more and more popular for protein engineering with the advantage of biological big-data. In this study, we described a method of rational design that is able to identify desired mutants by analyzing the coevolution of protein sequence. We employed this approach to evolve an archaeal isopentenyl phosphate kinase that can convert dimethylallyl alcohol (DMA) into precursor of isoprenoids. By designing 9 point mutations, we improved the catalytic activities of IPK about 8-fold in vitro. After introducing the optimal mutant of IPK into engineered E. coli strain for β-carotenoids production, we found that β-carotenoids production exhibited 97% increase over the starting strain. The process of enzyme optimization presented here could be used to improve the catalytic activities of other enzymes.
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