ArticleBiomicrofluidics2016
Electrical lysis of cells for detergent-free droplet assays.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Microfluidic Electroporation: Recent Advances and Applications.Advances in experimental medicine and biology · 2026Review
- Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands.ACS applied materials & interfaces · 2023Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.ACS synthetic biology · 2021Article
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Quantitative Approach for Protein Analysis in Small Cell Ensembles by an Integrated Microfluidic Chip with MALDI Mass Spectrometry.Analytical chemistry · 2021Article
- Prokaryotic single-cell RNA sequencing by in situ combinatorial indexing.Nature microbiology · 2020Article
- Electrochemical cell lysis of gram-positive and gram-negative bacteria: DNA extraction from environmental water samples.Electrochimica acta · 2020Article
- Recent advances in lab-on-a-chip technologies for viral diagnosis.Biosensors & bioelectronics · 2020Review
- Microfluidic single-cell analysis-Toward integration and total on-chip analysis.Biomicrofluidics · 2020Review
- Recent advances in microfluidic platforms for single-cell analysis in cancer biology, diagnosis and therapy.Trends in analytical chemistry : TRAC · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electric field immediately before encapsulation in droplets, resulting in cell lysis. We characterize lysis efficiency as a function of control parameters and demonstrate compatibility with enzymatic assays by measuring the catalysis of β-glucosidase, an important cellulase used in the conversion of biomass to biofuel. Our method enables assays in microfluidic droplets that are incompatible with detergents.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.