ArticleJournal of veterinary science2016
Production of α1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology.
Article in Journal of veterinary science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Histotripsy Ablation in Preclinical Animal Models of Cancer and Spontaneous Tumors in Veterinary Patients: A Review.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2022Review
- Current status of the application of gene editing in pigs.The Journal of reproduction and development · 2021Review
- Highly efficient multiplex genetic engineering of porcine primary fetal fibroblasts.Surgery open science · 2021Article
- Review
- Production of genetically modified pigs expressing human insulin and C-peptide as a source of islets for xenotransplantation.Transgenic research · 2019Article
- Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment.Journal of veterinary science · 2019Article
- Application of new biotechnologies for improvements in swine nutrition and pork production.Journal of animal science and biotechnology · 2019Review
- Generation of insulin-deficient piglets by disrupting INS gene using CRISPR/Cas9 system.Transgenic research · 2018Article
- Insight into the molecular mechanism of miR-192 regulatingBioscience reports · 2018Article
- Use of gene-editing technology to introduce targeted modifications in pigs.Journal of animal science and biotechnology · 2018Review
- Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.International journal of molecular sciences · 2017Article
- Generation of CMAHKO/GTKO/shTNFRI-Fc/HO-1 quadruple gene modified pigs.Transgenic research · 2017Article
- Production of heterozygous alpha 1,3-galactosyltransferase (GGTA1) knock-out transgenic miniature pigs expressing human CD39.Transgenic research · 2017Article
- Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs.Reproductive biology and endocrinology : RB&E · 2016Article
- Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases.Human genetics · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation. Human decay-accelerating factor (hDAF) gene, which can produce a complement inhibitor to protect cells from complement attack after xenotransplantation, was also integrated into the genome simultaneously. Plasmids coding for the TALEN pair and hDAF gene were transfected into porcine cells by electroporation to disrupt the porcine GGTA1 gene and express hDAF. The transfected cells were then sorted using a biotin-labeled IB4 lectin attached to magnetic beads to obtain GGTA1 deficient cells. As a result, we established GGTA1 knockout (KO) cell lines with biallelic modification (35.0%) and GGTA1 KO cell lines expressing hDAF (13.0%). When these cells were used for somatic cell nuclear transfer, we successfully obtained live GGTA1 KO pigs expressing hDAF. Our results demonstrate that TALEN-mediated genome editing is efficient and can be successfully used to generate gene edited pigs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.