ArticlePloS one2016
The Use of DNA Barcoding on Recently Diverged Species in the Genus Gentiana (Gentianaceae) in China.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Characterization and evolutionary insights into complete mitochondrial genome ofFrontiers in plant science · 2026Article
- De Novo assembly and phylogenetic analysis of the complete mitochondrial genome of Eleutherococcus senticosus and related araliaceous species.BMC plant biology · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Comparison of plastid genomes and ITS of two sister species in Gentiana and a discussion on potential threats for the endangered species from hybridization.BMC plant biology · 2023Article
- DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.Chinese medicine · 2022Review
- Sequencing and comparative analysis of chloroplast genomes of three medicinal plants:Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- DNA Barcoding and Phylogenomic Analysis of the GenusFrontiers in plant science · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- DNA barcoding of Oryza: conventional, specific, and super barcodes.Plant molecular biology · 2021Article
- Recurrent hybridization underlies the evolution of novelty inAoB PLANTS · 2021Article
- Discrimination ofMolecules (Basel, Switzerland) · 2020Article
- DNA barcodes for the identification of Stephania (Menispermaceae) species.Molecular biology reports · 2020Article
- DNA barcoding supports existence of morphospecies complex in endemic bamboo genusJournal of genetics · 2020Article
- The utility of DNA barcodes to confirm the identification of palm collections in botanical gardens.PloS one · 2020Article
- Comparative Chloroplast Genome Analyses of Species inInternational journal of molecular sciences · 2018Article
- Evaluation of single and multilocus DNA barcodes towards species delineation in complex tree genus Terminalia.PloS one · 2017Article
- Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera.PloS one · 2017Article
- Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes.Frontiers in plant science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA barcoding of plants poses particular challenges, especially in differentiating, recently diverged taxa. The genus Gentiana (Gentianaceae) is a species-rich plant group which rapidly radiated in the Himalaya-Hengduan Mountains in China. In this study, we tested the core plant barcode (rbcL + matK) and three promising complementary barcodes (trnH-psbA, ITS and ITS2) in 30 Gentiana species across 6 sections using three methods (the genetic distance-based method, Best Close Match and tree-based method). rbcL had the highest PCR efficiency and sequencing success (100%), while the lowest sequence recoverability was from ITS (68.35%). The presence of indels and inversions in trnH-psbA in Gentiana led to difficulties in sequence alignment. When using a single region for analysis, ITS exhibited the highest discriminatory power (60%-74.42%). Of the combinations, matK + ITS provided the highest discrimination success (71.43%-88.24%) and is recommended as the DNA barcode for the genus Gentiana. DNA barcoding proved effective in assigning most species to sections, though it performed poorly in some closely related species in sect. Cruciata because of hybridization events. Our analysis suggests that the status of G. pseudosquarrosa needs to be studied further. The utility of DNA barcoding was also verified in authenticating 'Qin-Jiao' Gentiana medicinal plants (G. macrophylla, G. crassicaulis, G. straminea, and G. dahurica), which can help ensure safe and correct usage of these well-known Chinese traditional medicinal herbs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.