ArticleBMC musculoskeletal disorders2016
Stem cells and bFGF in tendon healing: Effects of lentiviral gene transfer and long-term follow-up in a rat Achilles tendon defect model.
Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Tendon, Ligament, and Muscle Injury, Osteotendinous, Myotendinous, and Muscle-to-Bone Junction Therapy Perspectives with Growth Factors and Stable Gastric Pentadecapeptide BPC 157-A Review.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full-thickness tendon defect in a rat model.Clinics in shoulder and elbow · 2025Article
- FGF-2 enriched nanofiber scaffold for advancing achilles tendon healing: a comparative experimental investigation.Frontiers in surgery · 2024Article
- Comparison of mesenchymal stem cells from bone marrow, umbilical cord blood, and umbilical cord tissue in regeneration of a full-thickness tendon defectBiochemistry and biophysics reports · 2023Article
- Growth factors in the treatment of Achilles tendon injury.Frontiers in bioengineering and biotechnology · 2023Review
- New frontiers of tendon augmentation technology in tissue engineering and regenerative medicine: a concise literature review.The Journal of international medical research · 2022Review
- The Functions and Mechanisms of Basic Fibroblast Growth Factor in Tendon Repair.Frontiers in physiology · 2022Review
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Regeneration of a full-thickness defect of rotator cuff tendon with freshly thawed umbilical cord-derived mesenchymal stem cells in a rat model.Stem cell research & therapy · 2020Article
- Transcriptome profiling of digital flexor tendons after injury in a chicken model.Bioscience reports · 2020Article
- Tendon and Ligament Healing and Current Approaches to Tendon and Ligament Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- Regeneration of a full-thickness defect in rotator cuff tendon with umbilical cord-derived mesenchymal stem cells in a rat model.PloS one · 2020Article
- Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation.BMC musculoskeletal disorders · 2019Article
- Effects of transplantation of FGF-2-transfected MSCs and XACB on TNF-α expression with avascular necrosis of the femoral head in rabbits.Bioscience reports · 2019Article
- Stem cells as delivery vehicles for regenerative medicine-challenges and perspectives.World journal of stem cells · 2018Review
- Extracellular matrix protein production in human adipose-derived mesenchymal stem cells on three-dimensional polycaprolactone (PCL) scaffolds responds to GDF5 or FGF2.Gene reports · 2018Article
- Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair-An In Vitro Study.Stem cells international · 2018Article
- Non-invasive imaging of engineered human tumors in the living chicken embryo.Scientific reports · 2017Article
- Tendon injuries: Basic science and new repair proposals.EFORT open reviews · 2017Review
- Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture.BioMed research international · 2017Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe influence of stem cells and lentiviral expression of basic fibroblastic growth factor (bFGF) on tendon healing and remodelling was investigated in an in-vivo long-term (12 weeks) rat Achilles tendon defect model.
methodsIn sixty male Lewis rats, complete tendon defects (2.4 mm) were created and either left untreated (PBS) or treated by injection of stem cells lentivirally expressing the enhanced green fluorescence marker gene eGFP (MSC-LV-eGFP) or basic fibroblast growth factor bFGF (MSC-LV-bFGF). Tendons were harvested after 12 weeks and underwent biomechanical and (immuno)-histological analysis.
resultsAfter 12 weeks the mean ultimate load to failure ratio (treated side to contralateral side) in biomechanical testing reached 97 % in the bFGF-group, 103 % in the eGFP-group and 112 % in the PBS-group. Also in the stiffness testing both MSC groups did not reach the results of the PBS group. Histologically, the MSC groups did not show better results than the control group. There were clusters of ossifications found in all groups. In immunohistology, only the staining collagen-type-I was strongly increased in both MSC groups in comparison to PBS control group. However, there were no significant differences in the (immuno)-histological results between both stem cell groups.
conclusionThe biomechanical and (immuno)-histological results did not show positive effects of the MSC groups on tendon remodelling in a long-term follow-up. Interestingly, in later stages stem cells had hardly any effects on biomechanical results. This study inspires a critical and reflected use of stem cells in tendon healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.