ArticleRNA (New York, N.Y.)2016
Hormonal stimulation of starfish oocytes induces partial degradation of the 3' termini of cyclin B mRNAs with oligo(U) tails, followed by poly(A) elongation.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Recycling of Uridylated mRNAs in Starfish Embryos.Biomolecules · 2024Article
- Poly(U) polymerase activity in Caenorhabditis elegans regulates abundance and tailing of sRNA and mRNA.Genetics · 2024Article
- Cytoplasmic Polyadenylation Is an Ancestral Hallmark of Early Development in Animals.Molecular biology and evolution · 2023Article
- RNA uridylation and decay in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- MPF-based meiotic cell cycle control: Half a century of lessons from starfish oocytes.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus.PloS one · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
In yeast, plant, and mammalian somatic cells, short poly(A) tails on mRNAs are subject to uridylation, which mediates mRNA decay. Although mRNA uridylation has never been reported in animal oocytes, maternal mRNAs with short poly(A) tails are believed to be translationally repressed. In this study, we found that 96% of cyclin B mRNAs with short poly(A) tails were uridylated in starfish oocytes. Hormonal stimulation induced poly(A) elongation of cyclin B mRNA, and 62% of long adenine repeats did not contain uridine residues. To determine whether uridylated short poly(A) tails destabilize cyclin B mRNA, we developed a method for producing RNAs with the strict 3' terminal sequences of cyclin B, with or without oligo(U) tails. When we injected these synthetic RNAs into starfish oocytes prior to hormonal stimulation, we found that uridylated RNAs were as stable as nonuridylated RNAs. Following hormonal stimulation, the 3' termini of short poly(A) tails of synthesized RNAs containing oligo(U) tails were trimmed, and their poly(A) tails were subsequently elongated. These results indicate that uridylation of short poly(A) tails in cyclin B mRNA of starfish oocytes does not mediate mRNA decay; instead, hormonal stimulation induces partial degradation of uridylated short poly(A) tails in the 3'-5' direction, followed by poly(A) elongation. Oligo(U) tails may be involved in translational inactivation of mRNAs.
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