ArticleFrontiers in bioengineering and biotechnology2016
Cyclic Stretch Facilitates Myogenesis in C2C12 Myoblasts and Rescues Thiazolidinedione-Inhibited Myotube Formation.
Article in Frontiers in bioengineering and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Factors influencing thigh muscle volume change with cycling exercises in acute spinal cord injury - a secondary analysis of a randomized controlled trial.The journal of spinal cord medicine · 2022Trial
- Transcriptomic exploration unveils hub genes influencing fatty acid composition across various sections of yak meat.Genes & genomics · 2026Article
- In vivo mechano-tissue engineering by hydrogels capable of transmitting intercellular mechanical stress.Nature communications · 2025Article
- Current Methodologies for Inducing Aligned Myofibers in Tissue Constructs for Skeletal Muscle Tissue Regeneration.Advances in wound care · 2025Review
- Bioreactor development for skeletal muscle hypertrophy and atrophy by manipulating uniaxial cyclic strain: proof of concept.NPJ microgravity · 2024Article
- Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation.Bioengineering & translational medicine · 2024Article
- Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli.Science advances · 2023Article
- Directionality of developing skeletal muscles is set by mechanical forces.Nature communications · 2023Article
- Applying exercise-mimetic engineered skeletal muscle model to interrogate the adaptive response of irisin to mechanical force.iScience · 2022Article
- Modeling Patient-Specific Muscular Dystrophy Phenotypes and Therapeutic Responses in Reprogrammed Myotubes Engineered on Micromolded Gelatin Hydrogels.Frontiers in cell and developmental biology · 2022Article
- Impact of isolation method on cellular activation and presence of specific tendon cell subpopulations during in vitro culture.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Effects of mechanical trauma on the differentiation and ArfGAP3 expression of C2C12 myoblast and mouse levator ani muscle.International urogynecology journal · 2020Article
- Mechanical Stimulation of Adhesion Receptors Using Light-Responsive Nanoparticle Actuators Enhances Myogenesis.ACS applied materials & interfaces · 2020Article
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
- Targeted sequencing analysis of PPARG identifies a risk variant associated with obstructive sleep apnea in Chinese Han subjects.Sleep & breathing = Schlaf & Atmung · 2020Article
- Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers.Micromachines · 2019Article
- Stretching magnitude-dependent inactivation of AKT by ROS led to enhanced p53 mitochondrial translocation and myoblast apoptosis.Molecular biology of the cell · 2019Article
- Development of Microfluidic Stretch System for Studying Recovery of Damaged Skeletal Muscle Cells.Micromachines · 2018Article
- Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology.Scientific reports · 2018Article
- The Effect of Mechanical Loading Upon Extracellular Matrix Bioscaffold-Mediated Skeletal Muscle Remodeling.Tissue engineering. Part A · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10% strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco's modified Eagle's medium with 2% horse serum, facilitated myotube formation with increased myosin heavy chain and α-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs.
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