ArticleFrontiers in cellular and infection microbiology2016
Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages.
Article in Frontiers in cellular and infection microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 32 citations in OpenAlex.
- Selective Blockade of Two Aquaporin Channels, AQP3 and AQP9, Impairs Human Leukocyte Migration.Cells · 2025Article
- Mechanisms of the Quorum Sensing Systems ofCurrent medicinal chemistry · 2024Review
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- Role of aquaporins in corneal healing post chemical injury.Experimental eye research · 2023Review
- Aquaporins in Immune System.Advances in experimental medicine and biology · 2023Article
- Battle royale: Immune response on biofilms - host-pathogen interactions.Current research in immunology · 2023Review
- AQP9 transports lactate in tumor-associated macrophages to stimulate an M2-like polarization that promotes colon cancer progression.Biochemistry and biophysics reports · 2022Article
- An Organ System-Based Synopsis ofVirulence · 2021Review
- Gossip in the gut: Quorum sensing, a new player in the host-microbiota interactions.World journal of gastroenterology · 2021Review
- Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response.Cellular and molecular life sciences : CMLS · 2021Article
- Aquaporins in Immune Cells and Inflammation: New Targets for Drug Development.International journal of molecular sciences · 2021Review
- The Host Protein Aquaporin-9 is Required for EfficientFrontiers in cellular and infection microbiology · 2021Article
- Human Aquaporins: Functional Diversity and Potential Roles in Infectious and Non-infectious Diseases.Frontiers in genetics · 2021Review
- Surfactant phospholipids act as molecular switches for premature induction of quorum sensing-dependent virulence inVirulence · 2020Article
- Impact ofFrontiers in immunology · 2020Review
- Article
- Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of Pseudomonas aeruginosa Infections.Journal of innate immunity · 2019Review
- Dysregulation of schizophrenia-related aquaporin 3 through disruption of paranode influences neuronal viability.Journal of neurochemistry · 2018Article
- Perturbation of Wound Healing, Cytoskeletal Organization and Cellular Protein Networks during Hazara Virus Infection.Frontiers in cell and developmental biology · 2017Article
- Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quorum sensing (QS) communication allows Pseudomonas aeruginosa to collectively control its population density and the production of biofilms and virulence factors. QS signal molecules, like N-3-oxo-dodecanoyl-L-homoserine lactone (3O-C12-HSL), can also affect the behavior of host cells, e.g., by modulating the chemotaxis, migration, and phagocytosis of human leukocytes. Moreover, host water homeostasis and water channels aquaporins (AQP) are critical for cell morphology and functions as AQP interact indirectly with the cell cytoskeleton and signaling cascades. Here, we investigated how P. aeruginosa 3O-C12-HSL affects cell morphology, area, volume and AQP9 expression and distribution in human primary macrophages, using quantitative PCR, immunoblotting, two- and three-dimensional live imaging, confocal and nanoscale imaging. Thus, 3O-C12-HSL enhanced cell volume and area and induced cell shape and protrusion fluctuations in macrophages, processes tentatively driven by fluxes of water across cell membrane through AQP9, the predominant AQP in macrophages. Moreover, 3O-C12-HSL upregulated the expression of AQP9 at both the protein and mRNA levels. This was accompanied with enhanced whole cell AQP9 fluorescent intensity and redistribution of AQP9 to the leading and trailing regions, in parallel with increased cell area in the macrophages. Finally, nanoscopy imaging provided details on AQP9 dynamics and architecture within the lamellipodial area of 3O-C12-HSL-stimulated cells. We suggest that these novel events in the interaction between P. aeruginosa and macrophage may have an impact on the effectiveness of innate immune cells to fight bacteria, and thereby resolve the early stages of infections and inflammations.
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