ArticleFEBS open bio2016
Three-step procedure for preparation of pure Bacillus altitudinis ribonuclease.
Article in FEBS open bio, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Novel Organomineral Complex with Prolonged Antitumor Action.International journal of molecular sciences · 2025Article
- Bacillus secretes nucleases to degrade dsRNA, thereby reducing host's susceptibility to RNAi.NPJ biofilms and microbiomes · 2025Article
- Two Main Cancer Biomarkers as Molecular Targets of Binase Antitumor Activity.BioMed research international · 2024Article
- Fibrin-Rhamnogalacturonan I Composite Gel for Therapeutic Enzyme Delivery to Intestinal Tumors.International journal of molecular sciences · 2023Article
- Article
- Structural and Functional Differences between Homologous Bacterial Ribonucleases.International journal of molecular sciences · 2022Article
- The Cytotoxicity of RNase-Derived Peptides.Biomolecules · 2020Article
- Anti-Influenza Activity of the Ribonuclease Binase: Cellular Targets Detected by Quantitative Proteomics.International journal of molecular sciences · 2020Article
- Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids.Cells · 2020Article
- Zeolites as Carriers of Antitumor Ribonuclease Binase.Frontiers in pharmacology · 2019Article
- Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.Frontiers in immunology · 2019Review
- Bacterial ribonuclease binase exerts an intra-cellular anti-viral mode of action targeting viral RNAs in influenza a virus-infected MDCK-II cells.Virology journal · 2018Article
- The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly.BioMed research international · 2018Article
- Ribonuclease fromBioMed research international · 2017Article
- Novel Ribonuclease Activity Differs between Fibrillarins fromFrontiers in plant science · 2017Article
- A Novel Antiviral Strategy against MERS-CoV and HCoV-229E Using Binase to Target Viral Genome Replication.BioNanoScience · 2017Article
- Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells.Frontiers in pharmacology · 2017Article
- Article
- Extracellular Ribonuclease fromBioMed research internationalArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribonucleases are considered as promising tools for anticancer treatment due to their selective cytotoxicity against tumor cells. We investigated a new RNase from Bacillus altitudinis termed BALNASE (B. altitudinis RNase). Balnase is a close homolog of the well-known cytotoxic binase, differing by only one amino acid residue: nonpolar hydrophobic alanine at position 106 in the balnase molecule is replaced by a polar uncharged threonine in binase. The most exciting question is how the physico-chemical properties and biological effects of RNase might be changed by A106T substitution. Here, we have developed a chromatography-based rapid and modern technique for the purification of this new RNase which allowed us to get a protein sample of high quality with specific activity of 1.2 × 10(6) units in preparative amounts, suitable for further investigation of its biological properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.