ArticleFrontiers in neuroanatomy2016
A Simple and Efficient In Vivo Non-viral RNA Transfection Method for Labeling the Whole Axonal Tree of Individual Adult Long-Range Projection Neurons.
Article in Frontiers in neuroanatomy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Beyond Barrels: Diverse Thalamocortical Projection Motifs in the Mouse Ventral Posterior Complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Thalamic control of sensory processing and spindles in a biophysical somatosensory thalamoreticular circuit model of wakefulness and sleep.Cell reports · 2023Article
- Cerebellar and basal ganglia inputs define three main nuclei in the mouse ventral motor thalamus.Frontiers in neuroanatomy · 2023Article
- Benchmarking of tools for axon length measurement in individually-labeled projection neurons.PLoS computational biology · 2021Article
- Area-Specific Synapse Structure in Branched Posterior Nucleus Axons Reveals a New Level of Complexity in Thalamocortical Networks.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- A highly collateralized thalamic cell type with arousal-predicting activity serves as a key hub for graded state transitions in the forebrain.Nature neuroscience · 2018Article
- Connectomic Analysis of Brain Networks: Novel Techniques and Future Directions.Frontiers in neuroanatomy · 2016Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a highly efficient, simple, and non-infective method for labeling individual long-range projection neurons (LRPNs) in a specific location with enough sparseness and intensity to allow complete and unambiguous reconstructions of their entire axonal tree. The method is based on the "in vivo" transfection of a large RNA construct that drives the massive expression of green fluorescent protein. The method combines two components: injection of a small volume of a hyperosmolar NaCl solution containing the Pal-eGFP-Sindbis RNA construct (Furuta et al., 2001), followed by the application of high-frequency electric current pulses through the micropipette tip. We show that, although each component alone increases transfection efficacy, compared to simple volume injections of standard RNA solution, the highest efficacy (85.7%) is achieved by the combination of both components. In contrast with the infective viral Sindbis vector, RNA transfection occurs exclusively at the position of the injection micropipette tip. This method simplifies consistently labeling one or a few isolated neurons per brain, a strategy that allows unambiguously resolving and quantifying the brain-wide and often multi-branched monosynaptic circuits created by LRPNs.
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