ArticleFrontiers in neural circuits2016
Cerebellar Nuclei Neurons Show Only Small Excitatory Responses to Optogenetic Olivary Stimulation in Transgenic Mice: In Vivo and In Vitro Studies.
Article in Frontiers in neural circuits, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Developmentally Unique Cerebellar Processing Prioritizes Self- over Other-Generated Movements.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Cerebellum Lecture: the Cerebellar Nuclei-Core of the Cerebellum.Cerebellum (London, England) · 2024Review
- Developmentally unique cerebellar processing prioritizes self-over other-generated movements.bioRxiv : the preprint server for biology · 2024Article
- Complex spikes perturb movements and reveal the sensorimotor map of Purkinje cells.Current biology : CB · 2023Article
- Review
- Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive NetworkFrontiers in cellular neuroscience · 2022Article
- Activity of Cerebellar Nuclei Neurons Correlates with ZebrinII Identity of Their Purkinje Cell Afferents.Cells · 2021Article
- Population coding in the cerebellum: a machine learning perspective.Journal of neurophysiology · 2020Review
- Climbing fiber synapses rapidly and transiently inhibit neighboring Purkinje cells via ephaptic coupling.Nature neuroscience · 2020Article
- Article
- Cerebellar oscillations driven by synaptic pruning deficits of cerebellar climbing fibers contribute to tremor pathophysiology.Science translational medicine · 2020Article
- Emerging connections between cerebellar development, behaviour and complex brain disorders.Nature reviews. Neuroscience · 2019Review
- Dynamic modulation of activity in cerebellar nuclei neurons during pavlovian eyeblink conditioning in mice.eLife · 2017Article
- Synaptic excitation by climbing fibre collaterals in the cerebellar nuclei of juvenile and adult mice.The Journal of physiology · 2017Article
- In vivo analysis of synaptic activity in cerebellar nuclei neurons unravels the efficacy of excitatory inputs.The Journal of physiology · 2017Article
- Genetic silencing of olivocerebellar synapses causes dystonia-like behaviour in mice.Nature communications · 2017Article
- The Roles of the Olivocerebellar Pathway in Motor Learning and Motor Control. A Consensus Paper.Cerebellum (London, England) · 2017Article
- Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy.Frontiers in cellular neuroscience · 2017Article
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3 authors.
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Abstract
To study the olivary input to the cerebellar nuclei (CN) we used optogenetic stimulation in transgenic mice expressing channelrhodopsin-2 (ChR2) in olivary neurons. We obtained in vivo extracellular Purkinje cell (PC) and CN recordings in anesthetized mice while stimulating the contralateral inferior olive (IO) with a blue laser (single pulse, 10-50 ms duration). Peri-stimulus histograms (PSTHs) were constructed to show the spike rate changes after optical stimulation. Among 29 CN neurons recorded, 15 showed a decrease in spike rate of variable strength and duration, and only 1 showed a transient spiking response. These results suggest that direct olivary input to CN neurons is usually overridden by stronger PC inhibition triggered by climbing fiber responses. To further investigate the direct input from the climbing fiber collaterals we also conducted whole cell recordings in brain slices, where we used local stimulation with blue light. Due to the expression of ChR2 in PC axons as well as the IO in our transgenic line, strong inhibitory responses could be readily triggered with optical stimulation (13 of 15 neurons). After blocking this inhibition with GABAzine, only in 5 of 13 CN neurons weak excitatory responses were revealed. Therefore our in vitro results support the in vivo findings that the excitatory input to CN neurons from climbing fiber collaterals in adult mice is masked by the inhibition under normal conditions.
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