ArticleVeterinary world2015
Phenotypic and genotypic detection of virulence factors of Staphylococcus aureus isolated from clinical and subclinical mastitis in cattle and water buffaloes from different farms of Sadat City in Egypt.
Article in Veterinary world, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Prevalence of subclinical mastitis, its associated bacterial isolates and risk factors among cattle in Africa: a systematic review and meta-analysis.BMC veterinary research · 2023Pooled it
- "Just the Best Ones" combination: a new strategy for multi-epitope vaccine candidate based on immunoinformatics analysis to induce protective immunity against MRSA infection.Microbiology spectrum · 2026Article
- Genotypic detection of some virulence factors and genetic tree ofOpen veterinary journal · 2026Article
- Utilization of probiotics against virulence factors of Staphylococcus aureus in bovine mastitis.Antonie van Leeuwenhoek · 2025Review
- Comprehensive Molecular and Epidemiological Characterization ofPathogens (Basel, Switzerland) · 2025Article
- Article
- Molecular identification, virulence, and antibiotic-resistant genes characteristics ofOpen veterinary journal · 2024Article
- Virulence and resistance profiling of Staphylococcus aureus isolated from subclinical bovine mastitis in the Pakistani Pothohar region.Scientific reports · 2024Article
- Detection ofAnimals : an open access journal from MDPI · 2024Article
- On-farm epidemiology and phylogenetic evaluation of methicillin and beta-lactam-resistantIranian journal of veterinary research · 2024Article
- Article
- A report of coagulase-negativeIranian journal of veterinary research · 2024Article
- Occurrence of Virulence Genes among Methicillin-ResistantACS omega · 2023Article
- Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused byAnimals : an open access journal from MDPI · 2023Article
- Virulence genes and antibiotic resistance profiles ofIranian journal of veterinary research · 2023Article
- Antibiogram and virulence profiling reveals multidrug resistant Staphylococcus aureus as the predominant aetiology of subclinical mastitis in riverine buffaloes.Veterinary medicine and science · 2022Article
- Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives.BMC veterinary research · 2022Review
- Virulence and antimicrobial resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle.PloS one · 2022Article
- Sexually transmitted infections and semen quality from subfertile men with and without leukocytospermia.Reproductive biology and endocrinology : RB&E · 2021Article
- Characterization of Staphylococci and Streptococci Isolated from Milk of Bovides with Mastitis in Egypt.Pathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo characterize Staphylococcus aureus from clinical and subclinical mastitis and identify virulence factors. MATERIALS AND
methodsTwo hundred and two milk samples were collected, 143 from mastitic cattle and buffaloes 94 and 49, respectively, and 59 from apparently healthy cattle and buffaloes 35 and 24, respectively.
resultsCalifornia mastitis test was applied and positive prevalence were 91.48% and 75.51% for cattle and buffalo with clinical mastitis and 37.14% and 45.83% for cattle and buffalo with subclinical mastitis. S. aureus was isolated from clinically mastitic cattle and buffaloes were 39.29% and 50%, respectively. While, from subclinical mastitic cattle and buffaloes were 80% and 72.73%, respectively. Hemolytic activity was assessed for S. aureus isolated from clinically and subclinical mastitic cases with prevalences of 100% and 56.25%, respectively. Thermo nuclease production from clinically and subclinical mastitic cases was 25% and 56.25%, respectively. Simplex polymerase chain reaction (PCR) conducted on S. aureus using 16S rRNA, clumping factor A, Panton valentine leukocidin, coagulase (Coa), alpha-hemolysin and beta-hemolysin those proved existence in 100%, 46.9%, 65.6%, 100%, 34.4%, and 43.75% of the isolates, respectively. While, multiplex PCR is utilized for detection of enterotoxins and proved that 12.5% was positive for enterotoxine Type D.
conclusionsIt is concluded that simplex and multiplex PCR assays can be used as rapid and sensitive diagnostic tools to detect the presence of S. aureus and characterize its virulence factors that help in detection of severity of infection, distribution and stating preventive and control strategies.
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