Evidence map›Paper›PMID 27047075›Full record

ArticleVeterinary world2015

Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes.

Pankaj A Patel, Sandhya S Chaudhary, Gopal Puri, Virendra Kumar Singh, Arjun B Odedara

Open access · goldAbstract read
In one paragraph

Article in Veterinary world, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Pankaj A PatelDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India.
Sandhya S ChaudharyDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India.
Gopal PuriDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India.
Virendra Kumar SinghDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India.
Arjun B OdedaraDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India.
Navsari Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimThe present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes. MATERIALS AND

methodsOocytes were recovered from buffalo's ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vitro matured in maturation media supplemented with 0 μM (117 oocytes), 100 μM (46 oocytes) and 200 μM (42 oocytes) concentration of β-ME. After 24 h of incubation, maturation rate of oocytes and intra-cellular GSH level were determined.

resultsThe results showed that the presence of β-ME did not influence (p>0.05) the oocyte maturation rate. However, GSH level increased significantly (p<0.05) in matured oocytes when supplemented with 100 μM and 200 μM β-ME (6.19±0.10 and 6.37±0.20 pmol/oocyte) as compared to control media (4.68±0.26 pmol/oocyte).

conclusionIt was concluded that β-ME may have a potential to increase the meiotic maturation of in vitro cultured oocytes and protect it from oxidative damage.

Indexed as

buffalo oocytesglutathionein vitro maturationβ-mercaptoethanol

Identifiers

PMID27047075
PMCPMC4774706
OpenAlexW2155028580

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.