ArticleParasitology2016
No more time to stay 'single' in the detection of Anisakis pegreffii, A. simplex (s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach.
Article in Parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Developmental and geographic transcriptomic variation in Anisakis simplex (s. s.) reveals lncRNA-mediated regulation of mRNA expression.Scientific reports · 2026Article
- Genetic characterization ofInternational journal for parasitology. Parasites and wildlife · 2026Article
- Seasonal distribution of anisakid nematodes in fishes from Portuguese waters: Epidemiological update and implications to consumer risk.Current research in parasitology & vector-borne diseases · 2026Article
- Preliminary observations on muscle distribution ofFood and waterborne parasitology · 2025Article
- Anisakid biodiversity in two young harbour seals (Phoca vitulina L.) from coastal South-West Norway.Parasitology research · 2025Article
- Hybridization and introgression of the mitochondrial genome between the two speciesParasitology · 2025Article
- Anisakidae and Anisakidosis: A Public Health Perspective.Pathogens (Basel, Switzerland) · 2025Review
- Diversity, expression, and structural modeling of sugar transporters inFrontiers in cellular and infection microbiology · 2025Article
- New Insights on the Diversity, Ecology and Genetic Population Structure ofAnimals : an open access journal from MDPI · 2024Article
- Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1-3.EFSA journal. European Food Safety Authority · 2024Article
- Pericardial anisakiasis: unravelling diagnostic challenges in an unprecedented extra-abdominal manifestation: a case report.European heart journal. Case reports · 2024Article
- Modulation of the rat intestinal microbiota in the course ofFrontiers in veterinary science · 2024Article
- An update and ecological perspective on certain sentinel helminth endoparasites within the Mediterranean Sea.Parasitology · 2023Review
- Phylogenetic and phylogeographic analyses of Anisakis simplex sensu stricto (Nematoda: Anisakidae) from the common minke whale in Korean waters.Parasites, hosts and diseases · 2023Article
- Ascaridoid parasites in European sardine throughout the annual cycle: Variability in parasitic load according to host stock features.International journal for parasitology. Parasites and wildlife · 2023Article
- Population Genetic Structure ofAnimals : an open access journal from MDPI · 2023Article
- Genetic diversity ofFrontiers in veterinary science · 2023Article
- Metabolomic analysis reveals a differential adaptation process of the larval stages ofFrontiers in molecular biosciences · 2023Article
- Anisakid nematodes inFood and waterborne parasitology · 2022Article
- Distribution and genetic diversity of Anisakis spp. in cetaceans from the Northeast Atlantic Ocean and the Mediterranean Sea.Scientific reports · 2022Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multi-marker nuclear genotyping approach was performed on larval and adult specimens of Anisakis spp. (N = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species Anisakis pegreffii and Anisakis simplex (s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear DNA locus (i.e. partial sequence of the EF1 α-1 nDNA region) and (2) recognize hybrid categories. According to the Bayesian clustering algorithms, based on those markers, most of the individuals (N = 678) were identified as the parental species [i.e. A. pegreffii or A. simplex (s. s.)], whereas a smaller portion (N = 11) were recognized as F1 hybrids. Discordant results were obtained when using the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) on the same specimens, which indicated the occurrence of a large number of 'hybrids' both in sympatry and allopatry. These findings raise the question of possible misidentification of specimens belonging to the two parental Anisakis and their hybrid categories derived from the application of that single marker (i.e. PCR-RFLPs analysis of the ITS of rDNA). Finally, Bayesian clustering, using allozymes and EF1 α-1 nDNA markers, has demonstrated that hybridization between A. pegreffii and A. simplex (s. s.) is a contemporary phenomenon in sympatric areas, while no introgressive hybridization takes place between the two species.
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