ArticleScientific reports2016
Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Pooled it
- Pathogens as commensals: microbial priming of the immune system and heterologous protection.Microbiology (Reading, England) · 2026Review
- Streptococcus pneumoniae adaptation to nutrient deprivation and immune modulation drives upper respiratory tract colonization.The ISME journal · 2026Article
- Changes in carriage and serotype diversity of Streptococcus pneumoniae and other respiratory pathobionts in the UK between pre-PCV13 (2006-10), early-PCV13 (2010-12) and late-PCV13 (2012-23) periods.Pneumonia (Nathan Qld.) · 2025Article
- Temporal changes in pneumococcal carriage among childcare-attending children during the COVID-19 pandemic in the Greater New Haven Area, USA.Research square · 2025Article
- Article
- Temporal changes in pneumococcal carriage among childcare-attending children during the COVID-19 pandemic in the Greater New Haven Area, USA.medRxiv : the preprint server for health sciences · 2025Article
- Serological Profiling of Pneumococcal Proteins Reveals Unique Patterns of Acquisition, Maintenance, and Waning of Antibodies Throughout Life.The Journal of infectious diseases · 2024Article
- A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.Microbiology spectrum · 2024Article
- Contact with young children is a major risk factor for pneumococcal colonization in older adults.FEMS microbes · 2024Article
- Saliva as an alternative sample type for detection of pneumococcal carriage in young children.Microbiology (Reading, England) · 2023Article
- Highly Resistant Serotype 19A Streptococcus pneumoniae of the GPSC1/CC320 Clone from Invasive Infections in Poland Prior to Antipneumococcal Vaccination of Children.Infectious diseases and therapy · 2023Article
- Persistence of Pneumococcal Carriage among Older Adults in the Community despite COVID-19 Mitigation Measures.Microbiology spectrum · 2023Article
- High Levels of Detection of Nonpneumococcal Species of Streptococcus in Saliva from Adults in the United States.Microbiology spectrum · 2023Article
- Airway proteolytic control of pneumococcal competence.PLoS pathogens · 2023Article
- A spitting image: molecular diagnostics applied to saliva enhance detection ofFrontiers in microbiology · 2023Article
- Impact of Temporary Storage Conditions on the Viability of Streptococcus pneumoniae in Saliva.mSphere · 2022Article
- Trends in Asymptomatic NasopharyngealMicroorganisms · 2022Article
- Article
- Effect of Oral Streptococci Expressing Pneumococcus-like Cross-Reactive Capsule Types on World Health Organization Recommended Pneumococcal Carriage Detection Procedure.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following the introduction of pneumococcal conjugate vaccines (PCVs) for infants, surveillance studies on Streptococcus pneumoniae carriage have proven valuable for monitoring vaccine effects. Here, we compared molecular versus conventional diagnostic methods in prospective cross-sectional surveillances in vaccinated infants in the Netherlands. Nasopharyngeal samples (n = 1169) from 11- and 24-month-old children, collected during autumn/winter 2010/2011 and 2012/2013, were tested by conventional culture for S. pneumoniae. DNA extracted from all culture-plate growth was tested by qPCR for pneumococcal-specific genes (lytA/piaB) and selected serotypes (including PCV13-serotypes). qPCR significantly increased the number of carriers detected compared to culture (69% vs. 57%, p < 0.0001). qPCR assays targeting vaccine-serotypes 4 and 5 proved non-specific (results excluded). For serotypes reliably targeted by qPCR, the number of serotype-carriage events detected by qPCR (n = 709) was 1.68× higher compared to culture (n = 422). There was a strong correlation (rho = 0.980; p < 0.0001) between the number of serotypes detected using qPCR and by culture. This study demonstrates the high potential of molecular methods in pneumococcal surveillances, particularly for enhanced serotype detection. We found no evidence of a hidden circulation of vaccine-targeted serotypes, despite vaccine-serotypes still significantly contributing to invasive pneumococcal disease in unvaccinated individuals, supporting the presence of a substantial S. pneumoniae reservoir outside vaccinated children.
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