ArticleNucleic acids research1989
The 5' splice site: phylogenetic evolution and variable geometry of association with U1RNA.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed.
- Binding of RbFox proteins at weak 5'splice site of A2 induces its alternative splicing in non-muscle myosin heavy-chain IIA mRNA.The Journal of biological chemistry · 2026Article
- Multilayered control of splicing regulatory networks by DAP3 leads to widespread alternative splicing changes in cancer.Nature communications · 2022Article
- InnovativeFrontiers in genetics · 2022Review
- The evolution of hemocyanin genes in Tectipleura: a multitude of conserved introns in highly diverse gastropods.BMC ecology and evolution · 2021Article
- Genome-wide analyses supported by RNA-Seq reveal non-canonical splice sites in plant genomes.BMC genomics · 2018Article
- Genomic structure, expression and association study of the porcine FSD2.Molecular biology reports · 2016Article
- Analysis of mismatch repair gene mutations in Turkish HNPCC patients.Familial cancer · 2010Article
- Human Splicing Finder: an online bioinformatics tool to predict splicing signals.Nucleic acids research · 2009Article
- Human GC-AG alternative intron isoforms with weak donor sites show enhanced consensus at acceptor exon positions.Nucleic acids research · 2001Article
- Structure of the human biotinidase gene.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Analysis of donor splice sites in different eukaryotic organisms.Journal of molecular evolution · 1997Article
- Myomodulin gene of Lymnaea: structure, expression, and analysis of neuropeptides.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Molecular cloning and expression analysis of peroxidase genes from wheat.Plant molecular biology · 1995Article
- Interspecific sequence comparison of the muscle-myosin heavy-chain genes from Drosophila hydei and Drosophila melanogaster.Journal of molecular evolution · 1994Article
- Fission yeast gene structure and recognition.Nucleic acids research · 1994Article
- Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF.The EMBO journal · 1993Article
- Prenatal diagnosis and a donor splice site mutation in fibrillin in a family with Marfan syndrome.American journal of human genetics · 1993Article
- A T+6 to C+6 mutation in the donor splice site of COL3A1 IVS7 causes exon skipping and results in Ehlers-Danlos syndrome type IV.Journal of medical genetics · 1993Article
- Deletion of 19 base pairs in intron 13 of the gene for the pro alpha 2(I) chain of type-I procollagen (COL1A2) causes exon skipping in a proband with type-I osteogenesis imperfecta.Human genetics · 1993Article
- Factors affecting authentic 5' splice site selection in plant nuclei.Molecular and cellular biology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5' splice site sequences of 3294 introns from various organisms (1-672) were analyzed in order to determine the rules governing evolution of this sequence, which may shed light on the mechanism of cleavage at the exon-intron junction. The data indicate that, currently, in all organisms, a common sequence 1GUAAG6U and its derivatives are used as well as an additional sequence and its derivatives, which differ in metazoa (G/1GUgAG6U), lower eucaryotes (1GUAxG6U) and higher plants (AG/1GU3A). They all partly resemble the prototype sequence AG/1GUAAG6U whose 8 contigous nucleotides are complementary to the nucleotides 4-11 of U1RNA, which are perfectly conserved in the course of phylogenetic evolution. Detailed examination of the data shows that U1RNA can recognize different parts of 5' splice sites. As a rule, either prototype nucleotides at position -2 and -1 or at positions 4, 5 or 6 or at positions 3-4 are dispensable provided that the stability of the U1RNA-5' splice site hybrid is conserved. On the basis of frequency of sequences, the optimal size of the hybridizable region is 5-7 nucleotides. Thus, the cleavage at the exon-intron junction seems to imply, first, that the 5' splice site is recognized by U1RNA according to a "variable geometry" program; second, that the precise cleavage site is determined by the conserved sequence of U1RNA since it occurs exactly opposite to the junction between nucleotides C9 and C10 of U1RNA. The variable geometry of the U1RNA-5' splice site association provides flexibility to the system and allows diversification in the course of phylogenetic evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.