ArticleScientific reports2016
Optogenetic approaches addressing extracellular modulation of neural excitability.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Article
- Experimentally informed, quantitative photocycle model of the light-gated potassium channel WiChR.Biophysical journal · 2026Article
- Effects of optogenetic silencing the anterior cingulate cortex in a delayed non-match to trajectory task.Oxford open neuroscience · 2024Article
- Chronic Ethanol Exposure Modulates Periaqueductal Gray to Extended Amygdala Dopamine Circuit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology.International journal of molecular sciences · 2022Review
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022Article
- Photoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Activating Acid-Sensing Ion Channels with Photoacid Generators.ChemPhotoChem · 2020Article
- Parvalbumin and Somatostatin Interneurons Contribute to the Generation of Hippocampal Gamma Oscillations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- V1 interneurons regulate the pattern and frequency of locomotor-like activity in the neonatal mouse spinal cord.PLoS biology · 2019Article
- Comparative Principles for Next-Generation Neuroscience.Frontiers in behavioral neuroscience · 2019Article
- Ordered arrangement of dendrites within aeLife · 2018Article
- Electrical properties, substrate specificity and optogenetic potential of the engineered light-driven sodium pump eKR2.Scientific reports · 2018Article
- Advances in optogenetic and chemogenetic methods to study brain circuits in non-human primates.Journal of neural transmission (Vienna, Austria : 1996) · 2018Review
- Molecular determinants of proton selectivity and gating in the red-light activated channelrhodopsin Chrimson.Scientific reports · 2017Article
- Review
- Locus coeruleus to basolateral amygdala noradrenergic projections promote anxiety-like behavior.eLife · 2017Article
- Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extracellular ionic environment in neural tissue has the capacity to influence, and be influenced by, natural bouts of neural activity. We employed optogenetic approaches to control and investigate these interactions within and between cells, and across spatial scales. We began by developing a temporally precise means to study microdomain-scale interactions between extracellular protons and acid-sensing ion channels (ASICs). By coupling single-component proton-transporting optogenetic tools to ASICs to create two-component optogenetic constructs (TCOs), we found that acidification of the local extracellular membrane surface by a light-activated proton pump recruited a slow inward ASIC current, which required molecular proximity of the two components on the membrane. To elicit more global effects of activity modulation on 'bystander' neurons not under direct control, we used densely-expressed depolarizing (ChR2) or hyperpolarizing (eArch3.0, eNpHR3.0) tools to create a slow non-synaptic membrane current in bystander neurons, which matched the current direction seen in the directly modulated neurons. Extracellular protons played contributory role but were insufficient to explain the entire bystander effect, suggesting the recruitment of other mechanisms. Together, these findings present a new approach to the engineering of multicomponent optogenetic tools to manipulate ionic microdomains, and probe the complex neuronal-extracellular space interactions that regulate neural excitability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.