ArticleIEEE/ACM transactions on computational biology and bioinformatics
FocusALL: Focal Stacking of Microscopic Images Using Modified Harris Corner Response Measure.
Article in IEEE/ACM transactions on computational biology and bioinformatics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- EndoSCell: A portable intraoperative histopathological microscopy device for enhanced glioma resection and prognosis in animal models.APL bioengineering · 2025Article
- Focus stacking single-event particle radiography for high spatial resolution images and 3D feature localization.Physics in medicine and biology · 2024Article
- Automated, high-throughput quantification of EGFP-expressing neutrophils in zebrafish by machine learning and a highly-parallelized microscope.bioRxiv : the preprint server for biology · 2023Article
- Article
- Fast Multi-Focus Fusion Based on Deep Learning for Early-Stage Embryo Image Enhancement.Sensors (Basel, Switzerland) · 2021Article
- Protein Crystallization Segmentation and Classification Using Subordinate Color Channel in Fluorescence Microscopy Images.Journal of fluorescence · 2020Article
- Automated Drawing Tube (Camera Lucida) Method in Light Microscopy Images Analysis Can Comes True.Journal of microscopy and ultrastructureArticle
- Super-Thresholding: Supervised Thresholding of Protein Crystal Images.IEEE/ACM transactions on computational biology and bioinformaticsArticle
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Authors and funding
6 authors.
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Abstract
Automated image analysis of microscopic images such as protein crystallization images and cellular images is one of the important research areas. If objects in a scene appear at different depths with respect to the camera's focal point, objects outside the depth of field usually appear blurred. Therefore, scientists capture a collection of images with different depths of field. Focal stacking is a technique of creating a single focused image from a stack of images collected with different depths of field. In this paper, we introduce a novel focal stacking technique, FocusALL, which is based on our modified Harris Corner Response Measure. We also propose enhanced FocusALL for application on images collected under high resolution and varying illumination. FocusALL resolves problems related to the assumption that focus regions have high contrast and high intensity. Especially, FocusALL generates sharper boundaries around protein crystal regions and good in focus images for high resolution images in reasonable time. FocusALL outperforms other methods on protein crystallization images and performs comparably well on other datasets such as retinal epithelial images and simulated datasets.
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Registered trials
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