ArticleACS chemical biology2016
Global Isotope Metabolomics Reveals Adaptive Strategies for Nitrogen Assimilation.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
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- Growth, physiology, and metabolism ofFrontiers in microbiology · 2025Article
- The mitochondrial succinate dehydrogenase complex controls the STAT3-IL-10 pathway in inflammatory macrophages.iScience · 2023Article
- Metabolite discovery: Biochemistry's scientific driver.Cell metabolism · 2022Review
- Deciphering Microbial Metal Toxicity Responses via Random Bar Code Transposon Site Sequencing and Activity-Based Metabolomics.Applied and environmental microbiology · 2021Article
- Mechanism Across Scales: A Holistic Modeling Framework Integrating Laboratory and Field Studies for Microbial Ecology.Frontiers in microbiology · 2021Article
- METLIN: A Tandem Mass Spectral Library of Standards.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Defining and Evaluating Microbial Contributions to Metabolite Variation in Microbiome-Metabolome Association Studies.mSystems · 2019Article
- Systems-level analysis of isotopic labeling in untargeted metabolomic data by XNature protocols · 2019Article
- METLIN: A Technology Platform for Identifying Knowns and Unknowns.Analytical chemistry · 2018Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Nitrogen cycling is a microbial metabolic process essential for global ecological/agricultural balance. To investigate the link between the well-established ammonium and the alternative nitrate assimilation metabolic pathways, global isotope metabolomics was employed to examine three nitrate reducing bacteria using (15)NO3 as a nitrogen source. In contrast to a control (Pseudomonas stutzeri RCH2), the results show that two of the isolates from Oak Ridge, Tennessee (Pseudomonas N2A2 and N2E2) utilize nitrate and ammonia for assimilation concurrently with differential labeling observed across multiple classes of metabolites including amino acids and nucleotides. The data reveal that the N2A2 and N2E2 strains conserve nitrogen-containing metabolites, indicating that the nitrate assimilation pathway is a conservation mechanism for the assimilation of nitrogen. Co-utilization of nitrate and ammonia is likely an adaption to manage higher levels of nitrite since the denitrification pathways utilized by the N2A2 and N2E2 strains from the Oak Ridge site are predisposed to the accumulation of the toxic nitrite. The use of global isotope metabolomics allowed for this adaptive strategy to be investigated, which would otherwise not have been possible to decipher.
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Registered trials
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