ArticleScientific reports2016
Revealing a new activity of the human Dicer DUF283 domain in vitro.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Surface-enhanced Raman scattering detection of matrix metalloproteinase-9 via an aptamer-based plasmonic ruler.Analytical and bioanalytical chemistry · 2026Article
- Genome-Scale Identification, Phylogeny, Expression Profiling, and Functional Analysis of Sugarcane DUF4228 Family Involved in Drought Stress.Plants (Basel, Switzerland) · 2026Article
- Possible role of human ribonuclease dicer in the regulation of R loops.FEBS open bio · 2025Review
- Identification ofFrontiers in microbiology · 2025Article
- The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding.BMC biology · 2024Article
- DICER ribonuclease removes harmful R-loops.Molecular cell · 2023Article
- Research Progress in RNA-Binding Proteins.International journal of molecular sciences · 2022Article
- Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.Molecular cell · 2022Article
- Article
- RNA Interference: Promising Approach to Combat Plant Viruses.International journal of molecular sciences · 2022Review
- DICER: structure, function, and regulation.Biophysical reviews · 2021Review
- Dissecting protein domain variability in the core RNA interference machinery of five insect orders.RNA biology · 2021Article
- The Significance of the DUF283 Domain for the Activity of Human Ribonuclease Dicer.International journal of molecular sciences · 2021Article
- Article
- RNA and DNA G-quadruplexes bind to human dicer and inhibit its activity.Cellular and molecular life sciences : CMLS · 2021Article
- Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases.International journal of molecular sciences · 2021Review
- The RNA-RNA base pairing potential of human Dicer and Ago2 proteins.Cellular and molecular life sciences : CMLS · 2020Article
- Unknown Areas of Activity of Human Ribonuclease Dicer: A Putative Deoxyribonuclease Activity.Molecules (Basel, Switzerland) · 2020Article
- Review
- Dicer's Helicase Domain: A Meeting Place for Regulatory Proteins.Cold Spring Harbor symposia on quantitative biology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ribonuclease Dicer is a multidomain enzyme that plays a fundamental role in the biogenesis of small regulatory RNAs (srRNAs), which control gene expression by targeting complementary transcripts and inducing their cleavage or repressing their translation. Recent studies of Dicer's domains have permitted to propose their roles in srRNA biogenesis. For all of Dicer's domains except one, called DUF283 (domain of unknown function), their involvement in RNA substrate recognition, binding or cleavage has been postulated. For DUF283, the interaction with Dicer's protein partners has been the only function suggested thus far. In this report, we demonstrate that the isolated DUF283 domain from human Dicer is capable of binding single-stranded nucleic acids in vitro. We also show that DUF283 can act as a nucleic acid annealer that accelerates base-pairing between complementary RNA/DNA molecules in vitro. We further demonstrate an annealing activity of full length human Dicer. The overall results suggest that Dicer, presumably through its DUF283 domain, might facilitate hybridization between short RNAs and their targets. The presented findings reveal the complex nature of Dicer, whose functions may extend beyond the biogenesis of srRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.