ArticleThe Journal of cell biology2016
A versatile multivariate image analysis pipeline reveals features of Xenopus extract spindles.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Article
- Morphological growth dynamics, mechanical stability, and active microtubule mechanics underlying spindle self-organization.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling.The Journal of cell biology · 2022Article
- Spindle assembly in egg extracts of the Marsabit clawed frog, Xenopus borealis.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Subcellular scaling: does size matter for cell division?Current opinion in cell biology · 2018Review
- High-quality frozen extracts ofMolecular biology of the cell · 2017Article
- Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.Molecular biology of the cell · 2017Article
- Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus.The Journal of cell biology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Imaging datasets are rich in quantitative information. However, few cell biologists possess the tools necessary to analyze them. Here, we present a large dataset of Xenopusextract spindle images together with an analysis pipeline designed to assess spindle morphology across a range of experimental conditions. Our analysis of different spindle types illustrates how kinetochore microtubules amplify spindle microtubule density. Extract mixing experiments reveal that some spindle features titrate, while others undergo switch-like transitions, and multivariate analysis shows the pleiotropic morphological effects of modulating the levels of TPX2, a key spindle assembly factor. We also apply our pipeline to analyze nuclear morphology in human cell culture, showing the general utility of the segmentation approach. Our analyses provide new insight into the diversity of spindle types and suggest areas for future study. The approaches outlined can be applied by other researchers studying spindle morphology and adapted with minimal modification to other experimental systems.
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Registered trials
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