ArticleThe Journal of biological chemistry2016
Inorganic Phosphate Limitation Modulates Capsular Polysaccharide Composition in Mycobacteria.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Growth-rate dependent response of mycobacteria to relief of inhibition by a bacteriostatic antibiotic.FEMS microbiology letters · 2026Article
- Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids.Nature communications · 2025Article
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- Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations ofMethodsX · 2023Article
- SigE: A master regulator ofFrontiers in microbiology · 2023Review
- Insights into the molecular determinants involved inVirulence · 2021Review
- Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope.Chemical reviews · 2021Review
- Biofilms of the non-tuberculousCell surface (Amsterdam, Netherlands) · 2020Article
- Host Immune Response and Novel Diagnostic Approach to NTM Infections.International journal of molecular sciences · 2020Review
- Targeting Genome Integrity inMolecules (Basel, Switzerland) · 2020Review
- TheThe Biochemical journal · 2019Review
- Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages.BMC microbiology · 2018Article
- Mycobacteria employ two different mechanisms to cross the blood-brain barrier.Cellular microbiology · 2018Article
- The Alternative Sigma Factors SigE and SigB Are Involved in Tolerance and Persistence to Antitubercular Drugs.Antimicrobial agents and chemotherapy · 2017Article
- A fluorescence-based reporter for monitoring expression of mycobacterial cytochrome bd in response to antibacterials and during infection.Scientific reports · 2017Article
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- iniBAC induction Is Vitamin B12- and MutAB-dependent in Mycobacterium marinum.The Journal of biological chemistry · 2016Article
- Physiological Roles of the Dual Phosphate Transporter Systems in Low and High Phosphate Conditions and in Capsule Maintenance of Streptococcus pneumoniae D39.Frontiers in cellular and infection microbiology · 2016Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host. The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body. Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown. Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production. Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production. These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species. This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.