ArticleBMC microbiology2016
Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Molecular Profile and the Effectiveness of Antimicrobials Drugs AgainstInfection and drug resistance · 2023Article
- Identification of Three Type II Toxin-Antitoxin Systems in Model Bacterial Plant PathogenInternational journal of molecular sciences · 2021Article
- Transcriptional Organization of the Stability Module of Broad-Host-Range Plasmid RA3, from the IncU Group.Applied and environmental microbiology · 2020Article
- Molecular dissection of the replication system of plasmid pIGRK encoding two in-frame Rep proteins with antagonistic functions.BMC microbiology · 2019Article
- Reconsidering plasmid maintenance factors for computational plasmid design.Computational and structural biotechnology journal · 2019Review
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Authors and funding
7 authors.
Funding
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Abstract
backgroundLow-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use.
resultsTo facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation.
conclusionThe range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.
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